TissueScan, Breast Cancer cDNA Array IV

Snippet: …For example, IL-13 conjugated with Pseudomonas exotoxin subunits (IL-13;To determine relative IL-13Rα2 mRNA expression in breast cancer tumors representing various stages of the disease, a similar RT-PCR methodology was applied to Origene TissueScan Breast Cancer cDNA Arrays (samples from CSRT104, BCRT103, and BCRT104;60 tumor samples (n = 60) covering various disease stages with non-malignant (n = 7) breast tissue cDNA samples for comparison) [37]

Snippet: solation, cDNA synthesis and RT-qPCR were performed as described16,17 (Supplementary Table S1). RPLP0 was used as reference gene for RT-qPRs. Breast cancer cell lines and Breast Cancer cDNA array IV (Origene, BCRT104) were used as described16,17. Rapid amplification of cDNA ends (RACE)-specific cDNA synthesis was performed using the 3′ RACE Kit (Roche). Cells were treated with actinomycin D (Toc

Snippet: 1b, c). As mRNA expression data were not available for these cohorts, INPP4B mRNA expression was examined using Tissue Scan Breast Cancer cDNA arrays I-IV (OriGene) of 130 primary human breast cancers and 16 normal breast tissues (Fig. 1c);Breast cancer cDNA arrays (176 breast cancers and 16 tumor adjacent normal breast tissues) were purchased from OriGene (BCRT101, BCRT102, BCRT103, and BCRT104)

Snippet: To this end we examined PREX1 mRNA expression in TissueScan Human Breast Cancer cDNA arrays I–IV (OriGene), which contain 16 “normal” breast tissues and 176 primary breast cancer samples

Snippet: breast tissue samples. Briefly, TissueScan Cancer Survey Tissue qPCR panels BCRT101, BCRT102, BCRT103 and BCRT104 were arrayed onto a single 384‑well reaction plate by OriGene Technologies, Inc. Data are expressed

Snippet: TissueScan Disease Tissue qPCR Arrays (#BCRT104, Origene) were used to assess gene expression in 48 samples (4-normal, 2-Stage IA, 15-Stage IIA, 9-Stage IIB, 7-Stage IIIA, 4-Stage IIIB, 6-Stage IIIC, 1-Stage IV). Data was normalized to β-Actin expression and expressed relative to values obtained from normal tissue (N). Bars represent mean ± SEM. (D) GGCX protein expression in human breast tumors