TissueScan, Breast Cancer cDNA Array III

Snippet: >TXNIP expression using qPCR in 96 clinical samples across all stages of breast cancer, namely 5 normal epithelial, 23 stage I, 30 stage II, 29 stage III, and 9 stage IV metastatic biopsies (Origene, BCRT102, BCRT103), from which 90 samples yielded sufficient amount of cDNA. HPRT1 was used as an endogenous control and relative TXNIP expression levels were mea

Snippet: measured TXNIP expression using qPCR in 96 clinical samples across all stages of breast cancer, namely 5 normal epithelial, 23 stage I, 30 stage II, 29 stage III, and 9 stage IV metastatic biopsies (Origene, BCRT102, BCRT103), from which 90 samples yielded sufficient amount of cDNA. HPRT was used as an endogenous control and relative TXNIP expression levels were measured using the primers listed

Snippet: …For example, IL-13 conjugated with Pseudomonas exotoxin subunits (IL-13;To determine relative IL-13Rα2 mRNA expression in breast cancer tumors representing various stages of the disease, a similar RT-PCR methodology was applied to Origene TissueScan Breast Cancer cDNA Arrays (samples from CSRT104, BCRT103, and BCRT104;60 tumor samples (n = 60) covering various disease stages with non-malignant (n = 7) breast tissue cDNA samples for comparison) [37]

Snippet: of this family impact gene expression through different mechanisms, including DNA methylation13, chromatin modification14 and RNA degradation15. Some antisense RNAs have also been associated with tumorigenesis and a select few have a functional role in cancer progression16. Still, the extent to which antisense RNAs contribute to the regulation of gene expression in cancer is poorly understood. G;...

Snippet: at MCTS1 and SOD2 expression was positively correlated in breast cancer patients (p ;- Further characterization of SOD2 mRNA levels was conducted with breast cancer cDNA arrays (OriGene). SOD2 expression was enriched among stage II (3.57-fold) and advanced-stage (III/IV) (7.36-fold) tumor tissues (Fig. 1D) relative to normal breast tissues.

Snippet: 1b, c). As mRNA expression data were not available for these cohorts, INPP4B mRNA expression was examined using Tissue Scan Breast Cancer cDNA arrays I-IV (OriGene) of 130 primary human breast cancers and 16 normal breast tissues (Fig. 1c);Breast cancer cDNA arrays (176 breast cancers and 16 tumor adjacent normal breast tissues) were purchased from OriGene (BCRT101, BCRT102, BCRT103, and BCRT104)