IL5RA Human Recombinant Protein
Specifications
| Product Data | |
| Species | Human |
|---|---|
| Expression Host | HEK293 |
| Expression cDNA Clone or AA Sequence | |
| Tag | C-6×His Tag |
| Predicted MW | The protein has a predicted molecular mass of 37.0 kDa after removal of the signal peptide.The apparent molecular mass of IL5RA-His is approximately 50-55 kDa due to glycosylation. |
| Purity | The purity of the protein is greater than 90% as determined by SDS-PAGE and Coomassie blue staining. |
| Buffer | Lyophilized from sterile PBS, pH 7.4. Normally 5% - 8% trehalose is added as protectants before lyophilization |
| Reconstitution Method | 1. Before opening the tube cap, centrifuge the sample tube at 5000g for 3-5min at room temperature to ensure the lyophilized sample settles down at the bottom of the tube. 2. Calculate the volume for reconstitution (in µL) using the formula: Quantity (mg)/Concentration (mg/mL)x1000 3. Dissolve the lyophilized protein sample in sterile water based on the calculated volume (µL) 4. After adding sterile water, cover the lid and mix by gently tapping the tube 5-10 times. Note: Do not vortex or vigorously pipette the sample. |
| Preparation | Affinity purification |
| Storage | Store the lyophilized protein at -20°C. After reconstitution, store the protein at -80°C for 12 months. Avoid repeated freezing and thawing. |
| Stability | 12 months from date of despatch |
| Shipping | Ambient |
| Reference Data | |
| Locus ID | 3568 |
| UniProt ID | Q01344 |
| Synonyms | IL5Ra;CD125;IL-5 R alpha |
| Summary | The protein encoded by this gene is an interleukin 5 specific subunit of a heterodimeric cytokine receptor. The receptor is comprised of a ligand specific alpha subunit and a signal transducing beta subunit shared by the receptors for interleukin 3 (IL3), colony stimulating factor 2 (CSF2/GM-CSF), and interleukin 5 (IL5). The binding of this protein to IL5 depends on the beta subunit. The beta subunit is activated by the ligand binding, and is required for the biological activities of IL5. This protein has been found to interact with syndecan binding protein (syntenin), which is required for IL5 mediated activation of the transcription factor SOX4. Several alternatively spliced transcript variants encoding four distinct isoforms have been reported. |
| Protein Categories | CD Markers, Cytokines, Intracellular Proteins, Membrane Proteins |
| Protein Families | Druggable Genome, Transmembrane |
| Protein Pathways | Cytokine-cytokine receptor interaction, Hematopoietic cell lineage, Jak-STAT signaling pathway |
Reviews
Documents
| FAQs |
| SDS |
Resources
| Recombinant Protein Resources |
Other "IL5RA" proteins (12)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| PH305386 | IL5RA MS Standard C13 and N15-labeled recombinant protein (NP_783852) | 10 ug |
$3,360.00
|
|
| PH322553 | IL5RA MS Standard C13 and N15-labeled recombinant protein (NP_783854) | 10 ug |
$3,360.00
|
|
| LC403576 | Lyophilized IL5RA HEK293T cell transient overexpression lysate (as WB positive control) | 20 ug |
$206.00
|
|
| LC406266 | Lyophilized IL5RA HEK293T cell transient overexpression lysate (as WB positive control) | 20 ug |
$206.00
|
|
| LC406268 | Lyophilized IL5RA HEK293T cell transient overexpression lysate (as WB positive control) | 20 ug |
$206.00
|
|
| LC424642 | Lyophilized IL5RA HEK293T cell transient overexpression lysate (as WB positive control) | 20 ug |
$206.00
|
|
| LY403576 | Transient overexpression lysate of interleukin 5 receptor, alpha (IL5RA), transcript variant 6 (as WB positive control) | 100 ug |
$436.00
|
|
| LY406266 | Transient overexpression lysate of interleukin 5 receptor, alpha (IL5RA), transcript variant 3 (as WB positive control) | 100 ug |
$436.00
|
|
| LY406268 | Transient overexpression lysate of interleukin 5 receptor, alpha (IL5RA), transcript variant 5 (as WB positive control) | 100 ug |
$436.00
|
|
| LY424642 | Transient overexpression lysate of interleukin 5 receptor, alpha (IL5RA), transcript variant 1 (as WB positive control) | 100 ug |
$665.00
|
|
| TP305386 | Recombinant protein of human interleukin 5 receptor, alpha (IL5RA), transcript variant 3, 20 µg | 20 ug |
$737.00
|
|
| TP322553 | Purified recombinant protein of Homo sapiens interleukin 5 receptor, alpha (IL5RA), transcript variant 5, 20 µg | 20 ug |
$737.00
|
|
Citations
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