GPR75 Human Recombinant Protein, Synthetic Nanodisc

SKU TP721403M

Recombinant Human GPR75 full length protein-Synthetic Nanodisc, 100μg

Product Data

• Species: Human
• Expression Host: HEK293
• Expression cDNA Clone or AA Sequence:
  Protein Sequence

  C-terminal Flag tagged overexpression cDNA clone
• Tag: C-term Flag Tag
• Predicted MW: The human full length GPR75 protein has a MW of 59.4 kDa
• Concentration: Please refer to the Certificate of Analysis (COA) for the lot-specific concentration before lyophilization.
• Purity: The purity of the protein is determined by SDS-PAGE and Coomassie blue staining.
• Buffer: Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5% - 8% trehalose is added as protectants before lyophilization. Do not use solvents with pH lower than 6.5 in subsequent experiments.
• Reconstitution Method:
  1. Before opening the tube cap, centrifuge the sample tube at 5000g for 3-5min at room temperature to ensure the lyophilized sample settles down at the bottom of the tube.
  2. Calculate the volume for reconstitution (in µL) using the formula: [Quantity (mg)/Concentration (mg/mL)]x1000
  3. Dissolve the lyophilized protein sample in sterile water based on the calculated volume (µL)
  4. After adding sterile water, cover the lid and mix by gently tapping the tube 5-10 times. Note: Do not vortex or vigorously pipette the sample.
• Preparation: Affinity purification
• Storage: Store at -20°C to -80°C for 12 months in lyophilized form.
• Stability: After reconstitution, if not intended for use within a month, aliquot and store at -80°C . Avoid repeated freezing and thawing.
• Shipping: Ambient

Reference Data

• Locus ID: 10936
• UniProt ID: O95800
• Synonyms: GPRchr2; WI31133
• Protein Families: Druggable Genome, GPCR, Transmembrane
• Protein Pathways: Autoimmune thyroid disease, Neuroactive ligand-receptor interaction