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Negative Control, Synthetic Nanodisc

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SKU
TP721886
Negative Control-Synthetic Nanodisc, 10 ug
Size:
10 ug 100 ug
  $1,010.00
2 Weeks*
Bulk/Customize

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Specifications
Specifications
Product Data
Expression Host HEK293
Tag N-Flag & C-Strep Tag
Predicted MW 42.8 kDa
Concentration Please refer to the Certificate of Analysis (COA) for the lot-specific concentration before lyophilization.
Buffer Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5% – 8% trehalose is added as protectants before lyophilization. Do not use solvents with a pH below 6.5 or those containing high concentrations of divalent metal ions (greater than 5 mM) in subsequent experiments.
Reconstitution Method 1. Before opening the tube cap, centrifuge the sample tube at 5000g for 3-5min at room temperature to ensure the lyophilized sample settles down at the bottom of the tube.
2. Calculate the volume for reconstitution (in µL) using the formula: Quantity (mg)/Concentration (mg/mL)x1000
3. Dissolve the lyophilized protein sample in sterile water based on the calculated volume (µL)
4. After adding sterile water, cover the lid and mix by gently tapping the tube 5-10 times. Note: Do not vortex or vigorously pipette the sample.
Stability Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C . Avoid repeated freezing and thawing.
Shipping Ambient
Reference Data
Summary Nanodisc negative control is designed for use as a negative control for nanodisc membrane proteins. It provides a comparable nanodisc format without the target protein. The nanodisc contains a fusion protein with an N-terminal Flag tag, a PDGFRB-derived transmembrane region, and a C-terminal EGFP-Strep tag.
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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