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PERK (EIF2AK3) (NM_004836) Human Recombinant Protein

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SKU
TP314993
Recombinant protein of human eukaryotic translation initiation factor 2-alpha kinase 3 (EIF2AK3), 20 µg
Size:
20 ug 1 mg 100 ug
  $737.00
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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293
Expression cDNA Clone or AA Sequence
Protein Sequence
>RC214993 representing NM_004836
Red=Cloning site Green=Tags(s)

MERAISPGLLVRALLLLLLLLGLAARTVAAGRARGLPAPTAEAAFGLGAAAAPTSATRVPAAGAVAAAEV
TVEDAEALPAAAGEQEPRGPEPDDETELRPRGRSLVIISTLDGRIAALDPENHGKKQWDLDVGSGSLVSS
SLSKPEVFGNKMIIPSLDGALFQWDRDRESMETVPFTVESLLESSYKFGDDVVLVGGKSLTTYGLSAYSG
KVRYICSALGCRQWDSDEMEQEEDILLLQRTQKTVRAVGPRSGNEKWNFSVGHFELRYIPDMETRAGFIE
STFKPNENTEESKIISDVEEQEAAIMDIVIKVSVADWKVMAFSKKGGHLEWEYQFCTPIASAWLLKDGKV
IPISLFDDTSYTSNDDVLEDEEDIVEAARGATENSVYLGMYRGQLYLQSSVRISEKFPSSPKALESVTNE
NAIIPLPTIKWKPLIHSPSRTPVLVGSDEFDKCLSNDKFSHEEYSNGALSILQYPYDNGYYLPYYKRERN
KRSTQITVRFLDNPHYNKNIRKKDPVLLLHWWKEIVATILFCIIATTFIVRRLFHPHPHRQRKESETQCQ
TENKYDSVSGEANDSSWNDIKNSGYISRYLTDFEPIQCLGRGGFGVVFEAKNKVDDCNYAIKRIRLPNRE
LAREKVMREVKALAKLEHPGIVRYFNAWLEAPPEKWQEKMDEIWLKDESTDWPLSSPSPMDAPSVKIRRM
DPFSTKEHIEIIAPSPQRSRSFSVGISCDQTSSSESQFSPLEFSGMDHEDISESVDAAYNLQDSCLTDCD
VEDGTMDGNDEGHSFELCPSEASPYVRSRERTSSSIVFEDSGCDNASSKEEPKTNRLHIGNHCANKLTAF
KPTSSKSSSEATLSISPPRPTTLSLDLTKNTTEKLQPSSPKVYLYIQMQLCRKENLKDWMNGRCTIEERE
RSVCLHIFLQIAEAVEFLHSKGLMHRDLKPSNIFFTMDDVVKVGDFGLVTAMDQDEEEQTVLTPMPAYAR
HTGQVGTKLYMSPEQIHGNSYSHKVDIFSLGLILFELLYPFSTQMERVRTLTDVRNLKFPPLFTQKYPCE
YVMVQDMLSPSPMERPEAINIIENAVFEDLDFPGKTVLRQRSRSLSSSGTKHSRQSNNSHSPLPSN

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Tag C-Myc/DDK
Predicted MW 122.1 kDa
Concentration >0.05 µg/µL as determined by microplate BCA method
Purity > 80% as determined by SDS-PAGE and Coomassie blue staining
Buffer 25 mM Tris-HCl, 100 mM glycine, pH 7.3, 10% glycerol
Bioactivity Binding assay (ITC) (PMID: 28416388)
Preparation Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
Note For testing in cell culture applications, please filter before use. Note that you may experience some loss of protein during the filtration process.
Storage Store at -80°C.
Stability Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
Shipping Dry Ice
Reference Data
RefSeq NP_004827
Locus ID 9451
UniProt ID Q9NZJ5
Cytogenetics 2p11.2
RefSeq Size 4511
RefSeq ORF 3348
Synonyms PEK; PERK; WRS
Summary The protein encoded by this gene phosphorylates the alpha subunit of eukaryotic translation-initiation factor 2, leading to its inactivation, and thus to a rapid reduction of translational initiation and repression of global protein synthesis. This protein is thought to modulate mitochondrial function. It is a type I membrane protein located in the endoplasmic reticulum (ER), where it is induced by ER stress caused by malfolded proteins. Mutations in this gene are associated with Wolcott-Rallison syndrome. provided by RefSeq, Sep 2015
Protein Categories Diabetes, Enzyme: Kinases, Growth Factors, Intracellular Proteins, Membrane Proteins
Protein Families Druggable Genome, Protein Kinase, Secreted Protein, Transmembrane
Protein Pathways Alzheimer's disease
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Other "PERK" proteins (3)
SKU Description Size Price
PH314993 EIF2AK3 MS Standard C13 and N15-labeled recombinant protein (NP_004827) 10 ug
$3,360.00
LC417724 Lyophilized EIF2AK3 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LY417724 Transient overexpression lysate of eukaryotic translation initiation factor 2-alpha kinase 3 (EIF2AK3) (as WB positive control) 100 ug
$665.00

file-alt Citations

Human GRP78 affinity towards its signaling partners Ire1α and PERK is differently modulated by an unfolded protein client
Stan, Silva, de Camargo
Biochem. Biophys. Res. Commun. (2017) 487 (2), 375-380 DOI: 10.1016/j.bbrc.2017.04.069
AI Summary: OriGene provided purified human recombinant GRP78, Ire1α, and PERK proteins, which were essential for measuring binding affinities and investigating the interactions between these key proteins in the unfolded protein response studied in the paper.
Snippet: cancer). 2. Materials and methods. 2.1. Reagents. Purified human recombinant proteins GRP78, Ire1α and PERK expressed in HEK293 cells were purchased from OriGene (USA). LDH from rabbit muscle (EC 1.1.1.27) and sodium pyruvate were purchased from Sigma ... All 2 versions Cite SaveSaving
Showing 1-1 of 1 papers.
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