Glycerol kinase (GK) (NM_000167) Human Recombinant Protein

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SKU
TP313688
Recombinant protein of human glycerol kinase (GK), transcript variant 2, 20 µg
  $867.00
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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293
Expression cDNA Clone or AA Sequence
Protein Sequence
>RC213688 representing NM_000167
Red=Cloning site Green=Tags(s)

MAASKKAVLGPLVGAVDQGTSSTRFLVFNSKTAELLSHHQVEIKQEFPREGWVEQDPKEILHSVYECIEK
TCEKLGQLNIDISNIKAIGVSNQRETTVVWDKITGEPLYNAVVWLDLRTQSTVESLSKRIPGNNNFVKSK
TGLPLSTYFSAVKLRWLLDNVRKVQKAVEEKRALFGTIDSWLIWSLTGGVNGGVHCTDVTNASRTMLFNI
HSLEWDKQLCEFFGIPMEILPNVRSSSEIYGLMKAGALEGVPISGCLGDQSAALVGQMCFQIGQAKNTYG
TGCFLLCNTGHKCVFSDHGLLTTVAYKLGRDKPVYYALEGSVAIAGAVIRWLRDNLGIIKTSEEIEKLAK
EVGTSYGCYFVPAFSGLYAPYWEPSARGIICGLTQFTNKCHIAFAALEAVCFQTREILDAMNRDCGIPLS
HLQVDGGMTSNKILMQLQADILYIPVVKPSMPETTALGAAMAAGAAEGVGVWSLEPEDLSAVTMERFEPQ
INAEESEIRYSTWKKAVMKSMGWVTTQSPESGIP

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Tag C-Myc/DDK
Predicted MW 57.3 kDa
Concentration >0.05 µg/µL as determined by microplate BCA method
Purity > 80% as determined by SDS-PAGE and Coomassie blue staining
Buffer 25 mM Tris-HCl, 100 mM glycine, pH 7.3, 10% glycerol
Preparation Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
Note For testing in cell culture applications, please filter before use. Note that you may experience some loss of protein during the filtration process.
Storage Store at -80°C.
Stability Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
Shipping Dry Ice
Reference Data
RefSeq NP_000158
Locus ID 2710
UniProt ID P32189
Cytogenetics Xp21.2
RefSeq Size 3573
RefSeq ORF 1572
Synonyms GK1; GKD
Summary The protein encoded by this gene belongs to the FGGY kinase family. This protein is a key enzyme in the regulation of glycerol uptake and metabolism. It catalyzes the phosphorylation of glycerol by ATP, yielding ADP and glycerol-3-phosphate. Mutations in this gene are associated with glycerol kinase deficiency (GKD). Alternatively spliced transcript variants encoding different isoforms have been found for this gene. provided by RefSeq, Mar 2011
Protein Categories Intracellular Proteins, Membrane Proteins
Protein Families Druggable Genome
Protein Pathways Glycerolipid metabolism, Metabolic pathways, PPAR signaling pathway
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Other "Glycerol kinase" proteins (7)
SKU Description Size Price
PH307264 GK MS Standard C13 and N15-labeled recombinant protein (NP_976325) 10 ug
$3,360.00
PH313688 GK MS Standard C13 and N15-labeled recombinant protein (NP_000158) 10 ug
$3,360.00
LC404332 Lyophilized GK HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LC424888 Lyophilized GK HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LY404332 Transient overexpression lysate of glycerol kinase (GK), transcript variant 1 (as WB positive control) 100 ug
$436.00
LY424888 Transient overexpression lysate of glycerol kinase (GK), transcript variant 2 (as WB positive control) 100 ug
$436.00
TP307264 Recombinant protein of human glycerol kinase (GK), transcript variant 1, 20 µg 20 ug
$867.00

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