DNMT3A (NM_022552) Human Recombinant Protein

CAT#: TP313064

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Recombinant protein of human DNA (cytosine-5-)-methyltransferase 3 alpha (DNMT3A), transcript variant 3


 Product Datasheet for 'TP313064'

 Cited in 1 publication.

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USD 788.00


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    • 20 ug


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Specifications

Product Data
Description Recombinant protein of human DNA (cytosine-5-)-methyltransferase 3 alpha (DNMT3A), transcript variant 3
Species Human
Expression Host HEK293T
Tag C-Myc/DDK
Predicted MW 101.7 kDa
Concentration >50 ug/mL as determined by microplate BCA method
Purity > 80% as determined by SDS-PAGE and Coomassie blue staining
Buffer 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol
Bioactivity DNMT3A activity verified in a biochemical assay: DNMT3A (DNA (cytosine-5-)-methyltransferase 3 alpha) (TP313064) is a DNA methyltransferase that is thought to function in de novo methylation, rather than maintenance methylation. It modifies DNA in a non-processive manner and also methylates non-CpG sites. Varying concentrations of DNMT3A were added to a microplate containing a bound methyltransferase substrate. After incubation, the resulting methylated DNA residues were detected immunologically and a colorimetric signal was generated and measured.
Surface Plasmon Ressonance (SPR) (PMID: 27468168)
Preparation Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
Reference Data
RefSeq NP_072046
Locus ID 1788
Refseq Size 4324
Cytogenetics 2p23.3
Refseq ORF 2736
Synonyms DNMT3A2; M.HsaIIIA; TBRS
Summary CpG methylation is an epigenetic modification that is important for embryonic development, imprinting, and X-chromosome inactivation. Studies in mice have demonstrated that DNA methylation is required for mammalian development. This gene encodes a DNA methyltransferase that is thought to function in de novo methylation, rather than maintenance methylation. The protein localizes to the cytoplasm and nucleus and its expression is developmentally regulated. [provided by RefSeq, Mar 2016]
Protein Families Druggable Genome
Protein Pathways Cysteine and methionine metabolism, Metabolic pathways

Citations (1)

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