DNMT3A (NM_022552) Human Recombinant Protein
SKU
TP313064M
Recombinant protein of human DNA (cytosine-5-)-methyltransferase 3 alpha (DNMT3A), transcript variant 3, 100 µg
$2,508.00
4 Weeks*
Specifications
| Product Data | |
| Species | Human |
|---|---|
| Expression Host | HEK293 |
| Expression cDNA Clone or AA Sequence | |
| Tag | C-Myc/DDK |
| Predicted MW | 101.7 kDa |
| Concentration | >0.05 µg/µL as determined by microplate BCA method |
| Purity | > 80% as determined by SDS-PAGE and Coomassie blue staining |
| Buffer | 25 mM Tris-HCl, 100 mM glycine, pH 7.3, 10% glycerol |
| Bioactivity | DNMT3A activity verified in a biochemical assay: DNMT3A (DNA (cytosine-5-)-methyltransferase 3 alpha) (TP313064) is a DNA methyltransferase that is thought to function in de novo methylation, rather than maintenance methylation. It modifies DNA in a non-processive manner and also methylates non-CpG sites. Varying concentrations of DNMT3A were added to a microplate containing a bound methyltransferase substrate. After incubation, the resulting methylated DNA residues were detected immunologically and a colorimetric signal was generated and measured. Surface Plasmon Ressonance (SPR) (PMID: 27468168) |
| Preparation | Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps. |
| Note | For testing in cell culture applications, please filter before use. Note that you may experience some loss of protein during the filtration process. |
| Storage | Store at -80°C. |
| Stability | Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles. |
| Shipping | Dry Ice |
| Reference Data | |
| RefSeq | NP_072046 |
| Locus ID | 1788 |
| UniProt ID | Q9Y6K1 |
| Cytogenetics | 2p23.3 |
| RefSeq Size | 4324 |
| RefSeq ORF | 2736 |
| Synonyms | DNMT3A2; HESJAS; M.HsaIIIA; TBRS |
| Summary | CpG methylation is an epigenetic modification that is important for embryonic development, imprinting, and X-chromosome inactivation. Studies in mice have demonstrated that DNA methylation is required for mammalian development. This gene encodes a DNA methyltransferase that is thought to function in de novo methylation, rather than maintenance methylation. The protein localizes to the cytoplasm and nucleus and its expression is developmentally regulated. provided by RefSeq, Mar 2016 |
| Protein Categories | Cancer: Haematopoietic and Lymphoid, Cardiovascular diseases, Intracellular Proteins, Transciption Factors |
| Protein Families | Druggable Genome |
| Protein Pathways | Cysteine and methionine metabolism, Metabolic pathways |
Reviews
Documents
| FAQs |
| SDS |
Resources
| Recombinant Protein Resources |
Citations
DNA G-quadruplexes show strong interaction with DNA methyltransferases in vitro
Cree, Fredericks, Miller et al
FEBS Lett. (2016)
Cree, Fredericks, Miller et al
FEBS Lett. (2016)
Cited by 57
AI Summary: OriGene contributed to this study by providing purified DNA methyltransferase proteins, which were used to investigate the interaction between DNMTs and DNA G-quadruplexes in vitro.
Snippet: Measurement data were collected for an average of three scans over a range of 220–340 nm at 25 °C and baseline corrected (buffer only). Proteins. Purified DNA methyltransferase proteins were purchased from OriGene (Rockville, MD, USA). ... Cite SaveSaving
Showing 1-1 of 1 papers.
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen
complexities in the preparation of your product. International customers may expect an additional 1-2 weeks
in shipping.
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