NOTUM (NM_178493) Human Recombinant Protein
SKU
TP309331
Recombinant protein of human notum pectinacetylesterase homolog (Drosophila) (NOTUM), 20 µg
$737.00
In Stock*
Specifications
| Product Data | |
| Species | Human |
|---|---|
| Expression Host | HEK293 |
| Expression cDNA Clone or AA Sequence | |
| Tag | C-Myc/DDK |
| Predicted MW | 48.4 kDa |
| Concentration | >0.05 µg/µL as determined by microplate BCA method |
| Purity | > 80% as determined by SDS-PAGE and Coomassie blue staining |
| Buffer | 25 mM Tris-HCl, 100 mM glycine, pH 7.3, 10% glycerol |
| Bioactivity | Cell treatment (PMID: 29719227) |
| Preparation | Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps. |
| Note | For testing in cell culture applications, please filter before use. Note that you may experience some loss of protein during the filtration process. |
| Storage | Store at -80°C. |
| Stability | Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles. |
| Shipping | Dry Ice |
| Reference Data | |
| RefSeq | NP_848588 |
| Locus ID | 147111 |
| UniProt ID | Q6P988 |
| Cytogenetics | 17q25.3 |
| RefSeq Size | 2281 |
| RefSeq ORF | 1290 |
| Synonyms | hNOTUM |
| Summary | Carboxylesterase that acts as a key negative regulator of the Wnt signaling pathway by specifically mediating depalmitoleoylation of WNT proteins. Serine palmitoleoylation of WNT proteins is required for efficient binding to frizzled receptors (PubMed:25731175).UniProtKB/Swiss-Prot Function |
| Protein Categories | Intracellular Proteins, Secreated Proteins |
Reviews
Documents
| FAQs |
| SDS |
Resources
| Recombinant Protein Resources |
Other "NOTUM" proteins (3)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| PH309331 | NOTUM MS Standard C13 and N15-labeled recombinant protein (NP_848588) | 10 ug |
$3,360.00
|
|
| LC405887 | Lyophilized NOTUM HEK293T cell transient overexpression lysate (as WB positive control) | 20 ug |
$206.00
|
|
| LY405887 | Transient overexpression lysate of notum pectinacetylesterase homolog (Drosophila) (NOTUM) (as WB positive control) | 100 ug |
$436.00
|
|
Citations
A Strategy for Discovery of Endocrine Interactions with Application to Whole-Body Metabolism
Seldin, Koplev, Rajbhandari et al
Cell Metab. (2018) 27 (5), 1138-1155.e6 DOI: 10.1016/j.cmet.2018.03.015
Seldin, Koplev, Rajbhandari et al
Cell Metab. (2018) 27 (5), 1138-1155.e6 DOI: 10.1016/j.cmet.2018.03.015
AI Summary: OriGene’s human recombinant NOTUM protein was used to treat cells in vitro, enabling the researchers to investigate the effect of NOTUM on adipocyte gene expression and its role in inter-tissue endocrine signaling.
Snippet: After 48 hr, the cells were treated with a maintenance media containing the growth media supplemented with 5 mg/mL insulin, 1 nM 3,3’,5-Triiodo-L-thyronine (T3), and 0.5 mM Rosiglitazone, with a media change every 2-3 days until day 7 when RNA was isolated for gene expression analysis. For the NOTUM treatment group, 200 ng/mL of human recombinant NOTUM (Origene) were added to the induction media a...
Showing 1-1 of 1 papers.
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