UBE2L3 (NM_003347) Human Recombinant Protein

CAT#: TP308780

Recombinant protein of human ubiquitin-conjugating enzyme E2L 3 (UBE2L3), transcript variant 1, 20 µg

Product Images

The mean levels of serum ubiquitin-conjugating enzyme 2L3 autoantibody (UBE2L3-AAb) by age group in type 1 diabetes mellitus patients (squares) vs. non-diabetic control subjects (circles), as measured by ELISA using recombinant UBE2L3 (OriGene TP308780). a: significantly different compared to nondiabetic control subjects in each age group (P < 0.05); b: significantly different compared to 18- to 29-year-old in each disease status (non-diabetic control group and type 1 diabetes mellitus patients, respectively; P < 0.05). Figure cited from Diabetes Metab J, PMID: 26616590

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  Coomassie blue staining of purified UBE2L3 protein (Cat# TP308780). The protein was produced from HEK293T cells transfected with UBE2L3 cDNA clone (Cat# RC208780) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Species: Human
• Expression Host: HEK293T
• Expression cDNA Clone or AA Sequence:
  Protein Sequence

  >RC208780 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAASRRLMKELEEIRKCGMKNFRNIQVDEANLLTWQGLIVPDNPPYDKGAFRIEINFPAEYPFKPPKITF
  KTKIYHPNIDEKGQVCLPVISAENWKPATKTDQVIQSLIALVNDPQPEHPLRADLAEEYSKDRKKFCKNA
  EEFTKKYGEKRPVD
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Tag: C-Myc/DDK
• Predicted MW: 17.7 kDa
• Concentration: >0.05 µg/µL as determined by microplate BCA method
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Buffer: 25 mM Tris-HCl, 100 mM glycine, pH 7.3, 10% glycerol
• Bioactivity: ELISA capture for autoantibodies (PMID: 26616590)
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Note: For testing in cell culture applications, please filter before use. Note that you may experience some loss of protein during the filtration process.
• Storage: Store at -80°C.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.

Reference Data

• RefSeq: NP_003338
• Locus ID: 7332
• UniProt ID: P68036, A0A024R1A4
• Cytogenetics: 22q11.21
• Refseq Size: 3028
• Refseq ORF: 462
• Synonyms: E2-F1; L-UBC; UBCH7; UbcM4
• Summary: The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes (E1s), ubiquitin-conjugating enzymes (E2s) and ubiquitin-protein ligases (E3s). This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is demonstrated to participate in the ubiquitination of p53, c-Fos, and the NF-kB precursor p105 in vitro. Several alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Sep 2009]
• Protein Pathways: Parkinson's disease, Ubiquitin mediated proteolysis