ADO (NM_032804) Human Recombinant Protein

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SKU
TP305583
Recombinant protein of human 2-aminoethanethiol (cysteamine) dioxygenase (ADO), 20 µg
  $867.00
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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293
Expression cDNA Clone or AA Sequence
Protein Sequence
>RC205583 protein sequence
Red=Cloning site Green=Tags(s)

MPRDNMASLIQRIARQACLTFRGSWGGRGASDRDAASGAEAPMQPGFPENLSKLKSLLTQLRAEDLNIAP
RKATLQPLPPNLPPVTYMHIYETDGFSLGVFLLKSGTSIPLHDHPGMHGMLKVLYGTVRISCMDKLDAGG
GQRPRALPPEQQFEPPLQPREREAVRPGVLRSRAEYTEASGPCILTPHRDNLHQIDAVEGPAAFLDILAP
PYDPDDGRDCHYYRVLEPVRPKEASSSACDLPREVWLLETPQADDFWCEGEPYPGPKVFP

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Tag C-Myc/DDK
Predicted MW 29.6 kDa
Concentration >0.05 µg/µL as determined by microplate BCA method
Purity > 80% as determined by SDS-PAGE and Coomassie blue staining
Buffer 25 mM Tris-HCl, 100 mM glycine, pH 7.3, 10% glycerol
Preparation Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
Note For testing in cell culture applications, please filter before use. Note that you may experience some loss of protein during the filtration process.
Storage Store at -80°C.
Stability Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
Shipping Dry Ice
Reference Data
RefSeq NP_116193
Locus ID 84890
UniProt ID Q96SZ5
Cytogenetics 10q21.3
RefSeq Size 3739
RefSeq ORF 810
Synonyms C10orf22
Summary Human thiol dioxygenases include cysteine dioxygenase (CDO; MIM 603943) and cysteamine (2-aminoethanethiol) dioxygenase (ADO; EC 1.13.11.19). CDO adds 2 oxygen atoms to free cysteine, whereas ADO adds 2 oxygen atoms to free cysteamine to form hypotaurine (Dominy et al., 2007 PubMed 17581819).supplied by OMIM, Mar 2008
Protein Categories Intracellular Proteins
Protein Pathways Metabolic pathways, Taurine and hypotaurine metabolism
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Other "ADO" proteins (3)
SKU Description Size Price
PH305583 ADO MS Standard C13 and N15-labeled recombinant protein (NP_116193) 10 ug
$3,360.00
LC409931 Lyophilized ADO HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LY409931 Transient overexpression lysate of 2-aminoethanethiol (cysteamine) dioxygenase (ADO) (as WB positive control) 100 ug
$436.00

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