UBE2E2 (NM_152653) Human Recombinant Protein
SKU
TP304787
Recombinant protein of human ubiquitin-conjugating enzyme E2E 2 (UBC4/5 homolog, yeast) (UBE2E2), 20 µg
$737.00
In Stock*
Specifications
| Product Data | |
| Species | Human |
|---|---|
| Expression Host | HEK293 |
| Expression cDNA Clone or AA Sequence | |
| Tag | C-Myc/DDK |
| Predicted MW | 22.1 kDa |
| Concentration | >0.05 µg/µL as determined by microplate BCA method |
| Purity | > 80% as determined by SDS-PAGE and Coomassie blue staining |
| Buffer | 25 mM Tris-HCl, 100 mM glycine, pH 7.3, 10% glycerol |
| Preparation | Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps. |
| Note | For testing in cell culture applications, please filter before use. Note that you may experience some loss of protein during the filtration process. |
| Storage | Store at -80°C. |
| Stability | Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles. |
| Shipping | Dry Ice |
| Reference Data | |
| RefSeq | NP_689866 |
| Locus ID | 7325 |
| UniProt ID | Q96LR5 |
| Cytogenetics | 3p24.3 |
| RefSeq Size | 1760 |
| RefSeq ORF | 603 |
| Synonyms | UBCH8 |
| Summary | Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys-11'- and 'Lys-48'-, as well as 'Lys-63'-linked polyubiquitination. Catalyzes the ISGylation of influenza A virus NS1 protein.UniProtKB/Swiss-Prot Function |
| Protein Categories | Intracellular Proteins |
| Protein Pathways | Ubiquitin mediated proteolysis |
Reviews
Documents
| FAQs |
| SDS |
Resources
| Recombinant Protein Resources |
Other "UBE2E2" proteins (3)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| PH304787 | UBE2E2 MS Standard C13 and N15-labeled recombinant protein (NP_689866) | 10 ug |
$3,360.00
|
|
| LC407379 | Lyophilized UBE2E2 HEK293T cell transient overexpression lysate (as WB positive control) | 20 ug |
$206.00
|
|
| LY407379 | Transient overexpression lysate of ubiquitin-conjugating enzyme E2E 2 (UBC4/5 homolog, yeast) (UBE2E2) (as WB positive control) | 100 ug |
$436.00
|
|
Citations
Overexpression of UBE2E2 in mouse pancreatic β-cells leads to glucose intolerance via reduction of the β-cell mass
Sakurai, Kubota, Takamoto et al
Diabetes (2023)
Sakurai, Kubota, Takamoto et al
Diabetes (2023)
Cited by 5
AI Summary: OriGene’s recombinant human UBE2E2 protein (catalog number TP304787) was used in ubiquitination assays to study the role of UBE2E2 in polyubiquitin chain formation, helping to elucidate its impact on pancreatic β-cell function in the paper.
Snippet: 1 mM DTT, and 450 nM recombinant human UBE2E2 (ORIGENE, TP304787). After incubation at 37°C for 90 min, the reactions were stopped by the addition of SDS-PAGE sample buffer containing β-mercaptoethanol and boiling for 5 min, and resolved on 12 % SDS-polyacrylamide gels. Formation of polyubiquitin chains were evaluated by Western blot analysis with anti-Ubiquitin antibody
Showing 1-1 of 1 papers.
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