GI24 (C10orf54) (NM_022153) Human Recombinant Protein

CAT#: TP304547

Recombinant protein of human chromosome 10 open reading frame 54 (C10orf54), 20 µg

Product Images

Coomassie blue staining of purified VSIR protein (Cat# TP304547). The protein was produced from HEK293T cells transfected with VSIR cDNA clone (Cat# RC204547) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Species: Human
• Expression Host: HEK293T
• Expression cDNA Clone or AA Sequence:
  Protein Sequence

  >RC204547 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MGVPTALEAGSWRWGSLLFALFLAASLGPVAAFKVATPYSLYVCPEGQNVTLTCRLLGPVDKGHDVTFYK
  TWYRSSRGEVQTCSERRPIRNLTFQDLHLHHGGHQAANTSHDLAQRHGLESASDHHGNFSITMRNLTLLD
  SGLYCCLVVEIRHHHSEHRVHGAMELQVQTGKDAPSNCVVYPSSSQESENITAAALATGACIVGILCLPL
  ILLLVYKQRQAASNRRAQELVRMDSNIQGIENPGFEASPPAQGIPEAKVRHPLSYVAQRQPSESGRHLLS
  EPSTPLSPPGPGDVFFPSLDPVPDSPNFEVI
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Tag: C-Myc/DDK
• Predicted MW: 33.7 kDa
• Concentration: >0.05 µg/µL as determined by microplate BCA method
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Buffer: 25 mM Tris-HCl, 100 mM glycine, pH 7.3, 10% glycerol
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Note: For testing in cell culture applications, please filter before use. Note that you may experience some loss of protein during the filtration process.
• Storage: Store at -80°C.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.

Reference Data

• RefSeq: NP_071436
• Locus ID: 64115
• UniProt ID: Q9H7M9
• Cytogenetics: 10q22.1
• Refseq Size: 4774
• Refseq ORF: 933
• Synonyms: B7-H5; B7H5; C10orf54; DD1alpha; Dies1; GI24; PD-1H; PP2135; SISP1; VISTA
• Summary: Immunoregulatory receptor which inhibits the T-cell response (PubMed:24691993). May promote differentiation of embryonic stem cells, by inhibiting BMP4 signaling (By similarity). May stimulate MMP14-mediated MMP2 activation (PubMed:20666777).[UniProtKB/Swiss-Prot Function]
• Protein Families: Transmembrane