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SF3B5 (NM_031287) Human Recombinant Protein

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SKU
TP300977
Recombinant protein of human splicing factor 3b, subunit 5, 10kDa (SF3B5), 20 µg
Size:
20 ug 1 mg 100 ug
  $867.00
4 Weeks*
Bulk/Customize

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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293
Expression cDNA Clone or AA Sequence
Protein Sequence
>RC200977 protein sequence
Red=Cloning site Green=Tags(s)

MTDRYTIHSQLEHLQSKYIGTGHADTTKWEWLVNQHRDSYCSYMGHFDLLNYFAIAENESKARVRFNLME
KMLQPCGPPADKPEEN

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Tag C-Myc/DDK
Predicted MW 10 kDa
Concentration >0.05 µg/µL as determined by microplate BCA method
Purity > 80% as determined by SDS-PAGE and Coomassie blue staining
Buffer 25 mM Tris-HCl, 100 mM glycine, pH 7.3, 10% glycerol
Preparation Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
Note For testing in cell culture applications, please filter before use. Note that you may experience some loss of protein during the filtration process.
Storage Store at -80°C.
Stability Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
Shipping Dry Ice
Reference Data
RefSeq NP_112577
Locus ID 83443
UniProt ID Q9BWJ5
Cytogenetics 6q24.2
RefSeq Size 786
RefSeq ORF 258
Synonyms SF3b10; Ysf3
Summary Involved in pre-mRNA splicing as a component of the splicing factor SF3B complex, a constituent of the spliceosome (PubMed:27720643, PubMed:28781166). SF3B complex is required for 'A' complex assembly formed by the stable binding of U2 snRNP to the branchpoint sequence (BPS) in pre-mRNA. Sequence independent binding of SF3A/SF3B complex upstream of the branch site is essential, it may anchor U2 snRNP to the pre-mRNA (PubMed:12234937).UniProtKB/Swiss-Prot Function
Protein Categories Intracellular Proteins
Protein Pathways Spliceosome
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Other "SF3B5" proteins (3)
SKU Description Size Price
PH300977 SF3B5 MS Standard C13 and N15-labeled recombinant protein (NP_112577) 10 ug
$3,360.00
LC410578 Lyophilized SF3B5 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LY410578 Transient overexpression lysate of splicing factor 3b, subunit 5, 10kDa (SF3B5) (as WB positive control) 100 ug
$436.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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