CDK1 (NM_001786) Human Recombinant Protein

CAT#: TP300495

Recombinant protein of human cell division cycle 2, G1 to S and G2 to M (CDC2), transcript variant 1

Product images

Specifications

Product Data

• Description: Recombinant protein of human cell division cycle 2, G1 to S and G2 to M (CDC2), transcript variant 1
• Species: Human
• Expression Host: HEK293T
• Expression cDNA Clone or AA Sequence: Recombinant protein was produced with TrueORF clone, RC200495. Click on the TrueORF clone link to view cDNA and protein sequences.
• Tag: C-MYC/DDK
• Predicted MW: 33.9 kDa
• Concentration: >50 ug/mL as determined by microplate BCA method
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol. Store at –80C. Avoid repeated freeze-thaw cycles. Stable for 3 months from receipt of products under proper storage and handling conditions.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.

Reference Data

• RefSeq: NP_001777
• Locus ID: 983
• Refseq Size: 1923
• Cytogenetics: 10q21.2
• Refseq ORF: 894
• Synonyms: CDC2; CDC28A; P34CDC2
• Summary: The protein encoded by this gene is a member of the Ser/Thr protein kinase family. This protein is a catalytic subunit of the highly conserved protein kinase complex known as M-phase promoting factor (MPF), which is essential for G1/S and G2/M phase transitions of eukaryotic cell cycle. Mitotic cyclins stably associate with this protein and function as regulatory subunits. The kinase activity of this protein is controlled by cyclin accumulation and destruction through the cell cycle. The phosphorylation and dephosphorylation of this protein also play important regulatory roles in cell cycle control. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2009]
• Protein Families: Protein Kinase, Stem cell - Pluripotency, Druggable Genome
• Protein Pathways: Cell cycle, Oocyte meiosis, p53 signaling pathway, Gap junction, Progesterone-mediated oocyte maturation