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PEX2 (NM_001172087) Human Over-expression Lysate

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SKU
LY432857
Transient overexpression lysate of peroxisomal biogenesis factor 2 (PEX2), transcript variant 4 (as WB positive control)
  $436.00
5 Days*
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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC229857
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_001172087, NP_001165558
Synonyms PAF1; PBD5A; PBD5B; PMP3; PMP35; PXMP3; RNF72; ZWS3
Predicted MW 35.3 kDa
Components 1 vial of 100 µg total protein derived from gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
Storage The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 1x SDS-PAGE running buffer (with reducing agent). Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C.
Shipping Dry Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.).
Reference Data
RefSeq NP_001165558
Locus ID 5828
Cytogenetics 8q21.13
Protein Families WB
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Other "PEX2" proteins (3)
SKU Description Size Price
LC424799 Lyophilized PEX2 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LC432857 Lyophilized PEX2 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LY424799 Transient overexpression lysate of peroxisomal biogenesis factor 2 (PEX2), transcript variant 1 (as WB positive control) 100 ug
$436.00

file-alt Citations

Up-regulation of the Hippo pathway effector TAZ renders lung adenocarcinoma cells harboring EGFR-T790M mutation resistant to gefitinib
Xu, Wei, Wu et al
Cell Biosci (2015) 5, 7 DOI: 10.1186/2045-3701-5-7
Cited by 51
AI Summary: OriGene products contributed to this study by providing TAZ shRNA (TG311438) and TAZ cDNA (RC208374) plasmids, which were used to manipulate TAZ expression in lung adenocarcinoma cells to investigate its role in EGFR-TKI resistance.
FigureFigureFigure
Showing 1-1 of 1 papers.
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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