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P2X2 (P2RX2) (NM_174872) Human Over-expression Lysate

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SKU
LY430413
Transient overexpression lysate of purinergic receptor P2X, ligand-gated ion channel, 2 (P2RX2), transcript variant 5 (as WB positive control)
  $436.00
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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC219600
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_174872, NP_777361
Synonyms DFNA41; P2X2
Predicted MW 41.1 kDa
Components 1 vial of 100 µg total protein derived from gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
Storage The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 1x SDS-PAGE running buffer (with reducing agent). Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C.
Shipping Dry Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.).
Reference Data
RefSeq NP_777361
Locus ID 22953
Cytogenetics 12q24.33
Protein Families WB
Protein Pathways Calcium signaling pathway, Neuroactive ligand-receptor interaction
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Other "P2X2" proteins (18)
SKU Description Size Price
PH316207 P2RX2 MS Standard C13 and N15-labeled recombinant protein (NP_733782) 10 ug
$3,360.00
LC403528 Lyophilized P2RX2 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LC406287 Lyophilized P2RX2 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LC406792 Lyophilized P2RX2 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LC414051 Lyophilized P2RX2 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LC415901 Lyophilized P2RX2 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LC430303 Lyophilized P2RX2 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LC430413 Lyophilized P2RX2 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LY403528 Transient overexpression lysate of purinergic receptor P2X, ligand-gated ion channel, 2 (P2RX2), transcript variant 1 (as WB positive control) 100 ug
$665.00
LY406287 Transient overexpression lysate of purinergic receptor P2X, ligand-gated ion channel, 2 (P2RX2), transcript variant 5 (as WB positive control) 100 ug
$436.00
LY406792 Transient overexpression lysate of purinergic receptor P2X, ligand-gated ion channel, 2 (P2RX2), transcript variant 4 (as WB positive control) 100 ug
$665.00
LY414051 Transient overexpression lysate of purinergic receptor P2X, ligand-gated ion channel, 2 (P2RX2), transcript variant 3 (as WB positive control) 100 ug
$665.00
LY415901 Transient overexpression lysate of purinergic receptor P2X, ligand-gated ion channel, 2 (P2RX2), transcript variant 6 (as WB positive control) 100 ug
$436.00
LY430303 Transient overexpression lysate of purinergic receptor P2X, ligand-gated ion channel, 2 (P2RX2), transcript variant 4 (as WB positive control) 100 ug
$436.00
TP316207 Recombinant protein of human purinergic receptor P2X, ligand-gated ion channel, 2 (P2RX2), transcript variant 1, 20 µg 20 ug
$737.00
TP700189 Recombinant protein of human purinergic receptor P2X, ligand-gated ion channel, 2 (P2RX2), V60L mutant of transcript variant 1, 20 ug 20 ug
$900.00
TP700190 Recombinant protein of human purinergic receptor P2X, ligand-gated ion channel, 2 (P2RX2), K81A mutant of transcript variant 1, 20 ug 20 ug
$900.00
TP700191 Recombinant protein of human purinergic receptor P2X, ligand-gated ion channel, 2 (P2RX2), G353R mutant of transcript variant 1, 20 ug 20 ug
$900.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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