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LGR6 (NM_021636) Human Over-expression Lysate

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SKU
LY429655
Transient overexpression lysate of leucine-rich repeat-containing G protein-coupled receptor 6 (LGR6), transcript variant 2 (as WB positive control)
  $665.00
5 Days*
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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC208537
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_021636, NP_067649
Synonyms GPCR; VTS20631
Predicted MW 99.3 kDa
Components 1 vial of 100 µg total protein derived from gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
Storage The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 1x SDS-PAGE running buffer (with reducing agent). Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C.
Shipping Dry Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.).
Reference Data
RefSeq NP_067649
Locus ID 59352
Cytogenetics 1q32.1
Protein Families WB
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Other "LGR6" proteins (3)
SKU Description Size Price
LC422779 Lyophilized LGR6 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LC429655 Lyophilized LGR6 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LY422779 Transient overexpression lysate of leucine-rich repeat-containing G protein-coupled receptor 6 (LGR6), transcript variant 1 (as WB positive control) 100 ug
$665.00

file-alt Citations

Maresin 1 activates LGR6 receptor promoting phagocyte immunoresolvent functions
Chiang, Libreros, Norris et al
J. Clin. Invest. (2019) 129 (12), 5294-5311 DOI: 10.1172/JCI129448
AI Summary: OriGene products, specifically the mock vector pCMV6-AC-GFP (catalog no. PS100010) and the GFP-tagged human LGR6 expression plasmid (catalog no. RG208537), were used to transfect HEK-293 cells for functional assays assessing the activation of LGR6 by maresin 1 in this study.
Snippet: … HEK-293 cells were transfected with a mock vector (pCMV6-AC-GFP;catalog no. PS100010) or with an expression plasmid for human LGR6 (NM_021636;GFP-tagged;catalog no. RG208537;Origene). …
Showing 1-1 of 1 papers.
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