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TRIM66 (NM_014818) Human Over-expression Lysate

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SKU
LY429439
Transient overexpression lysate of tripartite motif-containing 66 (TRIM66) (as WB positive control)
  $665.00
5 Days*
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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC227892
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_014818, NP_055633
Synonyms C11orf29; TIF1D; TIF1DELTA
Predicted MW 134.5 kDa
Components 1 vial of 100 µg total protein derived from gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
Storage The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 1x SDS-PAGE running buffer (with reducing agent). Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C.
Shipping Dry Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.).
Reference Data
RefSeq NP_055633
Locus ID 9866
Cytogenetics 11p15.4
Protein Families WB
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Documents
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Other "TRIM66" proteins (1)
SKU Description Size Price
LC429439 Lyophilized TRIM66 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00

file-alt Citations

TRIM66 Overexpression Promotes Glioma Progression and Regulates Glucose Uptake Through cMyc/GLUT3 Signaling
Song, Meng, Yu et al
Cancer Manag Res (2021) 13, 5187-5201 DOI: 10.2147/CMAR.S293728
AI Summary: OriGene contributed to this study by providing the TRIM66 overexpression plasmid and negative control plasmid, which were used to investigate the role of TRIM66 in glioma progression and glucose uptake.
Snippet: Cells were cultured with mediums supplied with 10% fetal bovine serum (FBS). TRIM66 overexpression plasmid and negative control plasmid were purchased from Origene (Origene, Rockville, USA). Lipofectamine 3000 (Invitrogen, USA) was used for plasmid transfection
FigureFigureFigure
Showing 1-1 of 1 papers.
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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