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LAMC2 (NM_005562) Human Over-expression Lysate

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SKU
LY429255
Transient overexpression lysate of laminin, gamma 2 (LAMC2), transcript variant 1 (as WB positive control)
  $665.00
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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC222076
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_005562, NP_005553
Synonyms B2T; BM600; CSF; EBR2; EBR2A; LAMB2T; LAMNB2
Predicted MW 130.99 kDa
Components 1 vial of 100 µg total protein derived from gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
Storage The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 1x SDS-PAGE running buffer (with reducing agent). Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C.
Shipping Dry Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.).
Reference Data
RefSeq NP_005553
Locus ID 3918
Cytogenetics 1q25.3
Protein Families WB
Protein Pathways ECM-receptor interaction, Focal adhesion, Pathways in cancer, Small cell lung cancer
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Other "LAMC2" proteins (10)
SKU Description Size Price
PH318516 LAMC2 MS Standard C13 and N15-labeled recombinant protein (NP_061486) 10 ug
$3,360.00
PH322076 LAMC2 MS Standard C13 and N15-labeled recombinant protein (NP_005553) 10 ug
$3,360.00
LC412907 Lyophilized LAMC2 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LC417224 Lyophilized LAMC2 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LC429255 Lyophilized LAMC2 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LY412907 Transient overexpression lysate of laminin, gamma 2 (LAMC2), transcript variant 2 (as WB positive control) 100 ug
$665.00
LY417224 Transient overexpression lysate of laminin, gamma 2 (LAMC2), transcript variant 1 (as WB positive control) 100 ug
$665.00
TP318516 Recombinant protein of human laminin, gamma 2 (LAMC2), transcript variant 2, 20 µg 20 ug
$867.00
TP322076 Recombinant protein of human laminin, gamma 2 (LAMC2), transcript variant 1, 20 µg 20 ug
$867.00
TP762319 Purified recombinant protein of Human laminin, gamma 2 (LAMC2), transcript variant 1, Pro808-Leu909, with N-terminal His tag, expressed in E.coli, 50ug 50 ug
$260.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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