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Eph receptor A7 (EPHA7) (NM_004440) Human Over-expression Lysate

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SKU
LY429202
Transient overexpression lysate of EPH receptor A7 (EPHA7) (as WB positive control)
  $665.00
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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC226293
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_004440, NP_004431
Synonyms EHK-3; EHK3; EK11; HEK11
Predicted MW 111.9 kDa
Components 1 vial of 100 µg total protein derived from gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
Storage The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 1x SDS-PAGE running buffer (with reducing agent). Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C.
Shipping Dry Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.).
Reference Data
RefSeq NP_004431
Locus ID 2045
Cytogenetics 6q16.1
Protein Families WB
Protein Pathways Axon guidance
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Other "Eph receptor A7" proteins (5)
SKU Description Size Price
PH326293 EPHA7 MS Standard C13 and N15-labeled recombinant protein (NP_004431) 10 ug
$3,360.00
LC429202 Lyophilized EPHA7 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
TP326293 Recombinant protein of human EPH receptor A7 (EPHA7), 20 µg 20 ug
$867.00
TP700144 Purified recombinant protein of human EPH receptor A7 (EPHA7), with C-terminal DDK/His tag, expressed in human cells, 20 µg 20 ug
$900.00
TP710132 Recombinant protein of human EPH receptor A7 (EPHA7), residues 28-555aa, with C-terminal DDK tag, expressed in sf9, 20ug 20 ug
$540.00

file-alt Citations

Ligand-dependent EphA7 signaling inhibits prostate tumor growth and progression
Li, Wu, Ma et al
Cell Death Dis (2017) 8 (10), e3122 DOI: 10.1038/cddis.2017.507
AI Summary: OriGene’s human EphA7 cDNA (GenBank accession no. NM_004440.3) was used to generate wild-type and mutant EphA7 constructs, enabling the study of EphA7’s role in inhibiting prostate tumor growth and progression.
Snippet: … Human EphA7 (GenBank. … NM_004440.3) cDNAs were obtained from OriGene (Rockville, MD,. … EphA7 mutants were generated using the site-directed gene mutagenesis kit. …Human EphA7 (GenBank. … NM_004440.3) cDNAs were obtained from OriGene (Rockville, MD,. … EphA7 mutants were generated using the site-directed gene mutagenesis kit. …
Showing 1-1 of 1 papers.
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