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SATB1 (NM_001131010) Human Over-expression Lysate

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SKU
LY427355
Transient overexpression lysate of SATB homeobox 1 (SATB1), transcript variant 2 (as WB positive control)
  $665.00
5 Days*
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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC226152
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_001131010, NP_001124482
Synonyms DEFDA; KTZSL
Predicted MW 86 kDa
Components 1 vial of 100 µg total protein derived from gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
Storage The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 1x SDS-PAGE running buffer (with reducing agent). Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C.
Shipping Dry Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.).
Reference Data
RefSeq NP_001124482
Locus ID 6304
Cytogenetics 3p24.3
Protein Families WB
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Other "SATB1" proteins (7)
SKU Description Size Price
PH300421 SATB1 MS Standard C13 and N15-labeled recombinant protein (NP_002962) 10 ug
$3,360.00
PH326152 SATB1 MS Standard C13 and N15-labeled recombinant protein (NP_001124482) 10 ug
$3,360.00
LC418976 Lyophilized SATB1 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LC427355 Lyophilized SATB1 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LY418976 Transient overexpression lysate of SATB homeobox 1 (SATB1), transcript variant 1 (as WB positive control) 100 ug
$436.00
TP300421 Recombinant protein of human SATB homeobox 1 (SATB1), transcript variant 1, 20 µg 20 ug
$737.00
TP326152 Recombinant protein of human SATB homeobox 1 (SATB1), transcript variant 2, 20 µg 20 ug
$737.00

file-alt Citations

SATB1 is an independent prognostic factor in radically resected upper gastrointestinal tract adenocarcinoma
Hedner, Gaber, Korkocic et al
Virchows Arch. (2014)
Cited by 25
AI Summary: OriGene products SATB1 overexpression lysate (LY427355) and SATB2 overexpression lysate (LY414656) were used as positive controls in western blot analyses to validate antibody specificity in this study.
Snippet: blot analyses were performed according to standard protocols on SATB1 and SATB2 overexpression lysates co- expressed with a C-terminal myc-DDK tag (∼3.1 kDa) in mammalian HEK293T cells (LY427355 and LY414656, re- spectively, Origene Technologies, Rockville ... Cite SaveSaving
Showing 1-1 of 1 papers.
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