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PAK1 (NM_001128620) Human Over-expression Lysate

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SKU
LY426982
Transient overexpression lysate of p21 protein (Cdc42/Rac)-activated kinase 1 (PAK1), transcript variant 1 (as WB positive control)
  $436.00
5 Days*
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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC225947
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_001128620, NP_001122092
Synonyms alpha-PAK; IDDMSSD; p65-PAK; PAKalpha
Predicted MW 61.5 kDa
Components 1 vial of 100 µg total protein derived from gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
Storage The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 1x SDS-PAGE running buffer (with reducing agent). Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C.
Shipping Dry Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.).
Reference Data
RefSeq NP_001122092
Locus ID 5058
Cytogenetics 11q13.5-q14.1
Protein Families WB
Protein Pathways Axon guidance, Chemokine signaling pathway, Epithelial cell signaling in Helicobacter pylori infection, ErbB signaling pathway, Fc gamma R-mediated phagocytosis, Focal adhesion, MAPK signaling pathway, Natural killer cell mediated cytotoxicity, Regulation of actin cytoskeleton, Renal cell carcinoma, T cell receptor signaling pathway
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Other "PAK1" proteins (5)
SKU Description Size Price
PH325947 PAK1 MS Standard C13 and N15-labeled recombinant protein (NP_001122092) 10 ug
$3,360.00
LC426982 Lyophilized PAK1 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
TP325947 Recombinant protein of human p21 protein (Cdc42/Rac)-activated kinase 1 (PAK1), transcript variant 1, 20 µg 20 ug
$867.00
TP723901 Purified recombinant kinase domain protein of human p21 protein (Cdc42/Rac)-activated kinase 1 (PAK1), transcript variant 1, 10 ug 10 ug
$820.00
TP723902 Purified recombinant kinase domain protein of human p21 protein (Cdc42/Rac)-activated kinase 1 (PAK1), transcript variant 1, and phosphorylated at amino acid T423, 100 ug 100 ug
$2,845.00

file-alt Citations

PAK1 confers chemoresistance and poor outcome in non-small cell lung cancer via β-catenin-mediated stemness
Chen, Wu, Wang et al
Sci Rep (2016) 6, 34933 DOI: 10.1038/srep34933
AI Summary: OriGene's PAK1-overexpression plasmid was used in this study to experimentally increase PAK1 levels in non-small cell lung cancer cells, enabling the authors to investigate PAK1's role in chemoresistance and stemness.
Snippet: Plasmid constructs and transfection. The PAK1-overexpression plasmidwas purchased from Origene (Rockville, MD). The -catenin-overexpression plasmidwas purchased from Addgene (Addgene Company, Cambridge). ... Cite SaveSaving
FigureFigureFigure
Showing 1-1 of 1 papers.
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