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Plasminogen (PLG) (NM_000301) Human Over-expression Lysate

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SKU
LY424815
Transient overexpression lysate of plasminogen (PLG), transcript variant 1 (as WB positive control)
  $436.00
2 Weeks*
Bulk/Customize

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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC208871
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_000301, NP_000292
Synonyms HAE4
Predicted MW 90.6 kDa
Components 1 vial of 100 µg total protein derived from gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
Storage The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 1x SDS-PAGE running buffer (with reducing agent). Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C.
Shipping Dry Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.).
Reference Data
RefSeq NP_000292
Locus ID 5340
Cytogenetics 6q26
Protein Families WB
Protein Pathways Complement and coagulation cascades, Neuroactive ligand-receptor interaction
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Other "Plasminogen" proteins (4)
SKU Description Size Price
LC424815 Lyophilized PLG HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
TP762039 Purified recombinant protein of Human plasminogen (PLG), transcript variant 1,Ala301-Val586, with N-terminal His tag, expressed in E. coli, 50ug 50 ug
$270.00
TP762645 Purified recombinant protein of Human plasminogen (PLG), transcript variant 1, full length, with N-GST and C-His tag, expressed in E.coli, 50ug 50 ug
$270.00
TP762698 Purified recombinant protein of Human plasminogen (PLG), transcript variant 1, Glu20-End, with C-terminal His tag, expressed in E.coli, 50ug 50 ug
$270.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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