TAT (NM_000353) Human Over-expression Lysate

CAT#: LY424771

Transient overexpression lysate of tyrosine aminotransferase (TAT), nuclear gene encoding mitochondrial protein

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LY424771 is the updated version of LC424771.

USD 495.00


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Size
    • 100 ug

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Specifications

Product Data
Description Transient overexpression lysate of tyrosine aminotransferase (TAT), nuclear gene encoding mitochondrial protein
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC216782
Tag C-MYC/DDK
Detection Antibodies Anti-DDK Antibody (TA50011-100)
Accession Number NM_000353, NP_000344
Other Names TAT, tyrosine aminotransferase, cytosolic; tyrosine aminotransferase
Predicted MW 50.2 kDa
Components 1 vial of 100 µg gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg empty vector transfected control cell lysate in RIPA buffer
1 vial of 250ul 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromphenol blue, 100mM DTT)
Storage The lysate is shipped with dry ice. Upon receiving, store the sample at -20ºC. Lysate samples are stable for 12 months from date of receipt when stored at -20ºC. Avoid repeated freeze-thaw cycles.
Lysate samples can be diluted with 2xSDS Sample Buffer provided. After dilution, the protein sample should be aliquoted and stored at -20ºC for long term storage. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes.
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). Cell lysates were aliquoted and stored at -20ºC before shipping.
Reference Data
RefSeq NP_000344
Locus ID 6898
Cytogenetics tyrosine aminotransferase, cytosolic; tyrosine aminotransferase
Protein Families ES Cell Differentiation/IPS, Embryonic stem cells, Druggable Genome
Protein Pathways Ubiquinone and other terpenoid-quinone biosynthesis, Cysteine and methionine metabolism, Tyrosine metabolism, Phenylalanine metabolism, Phenylalanine, tyrosine and tryptophan biosynthesis, Metabolic pathways
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.