HTR1B (NM_000863) Human Over-expression Lysate
SKU
LY424477
Transient overexpression lysate of 5-hydroxytryptamine (serotonin) receptor 1B (HTR1B) (as WB positive control)
$436.00
5 Days*
Specifications
| Product Data | |
| Species | Human |
|---|---|
| Expression Host | HEK293T |
| Expression cDNA Clone or AA Sequence | TrueORF Clone RC223874 |
| Tag | C-Myc/DDK |
| Detection Antibodies | Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100) |
| ACCN | NM_000863, NP_000854 |
| Synonyms | 5-HT-1B; 5-HT-1D-beta; 5-HT1B; 5-HT1DB; HTR1D2; HTR1DB; S12 |
| Predicted MW | 43.4 kDa |
| Components | 1 vial of 100 µg total protein derived from gene specific transient over-expression cell lysate in RIPA buffer 1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer |
| Storage | The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 1x SDS-PAGE running buffer (with reducing agent). Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. |
| Shipping | Dry Ice |
| Preparation | HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). |
| Reference Data | |
| RefSeq | NP_000854 |
| Locus ID | 3351 |
| Cytogenetics | 6q14.1 |
| Protein Families | WB |
| Protein Pathways | Neuroactive ligand-receptor interaction |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
| Recombinant Protein Resources |
Other "HTR1B" proteins (1)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| LC424477 | Lyophilized HTR1B HEK293T cell transient overexpression lysate (as WB positive control) | 20 ug |
$206.00
|
|
Citations
The Role of Serotonin in Regulating Mucosal Epithelial Barrier in Inflammatory Bowel Disease
Johnson
Thesis (2025)
Johnson
Thesis (2025)
AI Summary: OriGene's over-expression cell lysate for HTR1B (LC424477) was used as a Western blot detection control in this study investigating serotonin's role in regulating the mucosal epithelial barrier in inflammatory bowel disease.
Snippet: nd IRDye 680RD Goat anti-Rabbit IgG (Licor Bio, 926-68071). After washing with PBS-T, protein bands were detected using the Odyssey Infrared Imaging System. Densitometric analysis was performed using ImageJ software. Over-expression cell lysate for HTR1B was purchased from Origene as a WB detection control (LC424477)
Showing 1-1 of 1 papers.
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen
complexities in the preparation of your product. International customers may expect an additional 1-2 weeks
in shipping.
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