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PRIM1 (NM_000946) Human Over-expression Lysate

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SKU
LY424441
Transient overexpression lysate of primase, DNA, polypeptide 1 (49kDa) (PRIM1) (as WB positive control)
  $436.00
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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC202710
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_000946, NP_000937
Synonyms p49
Predicted MW 49.9 kDa
Components 1 vial of 100 µg total protein derived from gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
Storage The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 1x SDS-PAGE running buffer (with reducing agent). Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C.
Shipping Dry Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.).
Reference Data
RefSeq NP_000937
Locus ID 5557
Cytogenetics 12q13.3
Protein Families WB
Protein Pathways DNA replication, Metabolic pathways, Purine metabolism, Pyrimidine metabolism
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Documents
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Other "PRIM1" proteins (1)
SKU Description Size Price
LC424441 Lyophilized PRIM1 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00

file-alt Citations

Redox-driven ADAR1 activation promotes Okazaki fragment maturation and DNA replication integrity
Chen, Sun, Kloeber et al
Nat Struct Mol Biol (2026) 33 (2), 293-303 DOI: 10.1038/s41594-025-01736-w
Cited by 3
AI Summary: OriGene products—MYC/DDK-PCNA human recombinant protein (TP301741), MYC/DDK-POLD1 human overexpression lysate (LY400947), and c-MYC/DDK-PRIM1 human overexpression lysate (LY424441)—were used in this study to investigate the molecular mechanisms underlying ADAR1-mediated RNA editing and Okazaki fragment maturation during DNA replication.
Snippet: The MYC/DDK-PCNA human recombinant protein (TP301741), MYC/DDK-POLD1 human overexpression lysate (LY400947) and c-MYC/DDK-PRIM1 human overexpression lysate (LY424441) were purchased from Origene
Showing 1-1 of 1 papers.
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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