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Cardiac Troponin T (TNNT2) (NM_001001430) Human Over-expression Lysate

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SKU
LY424349
Transient overexpression lysate of troponin T type 2 (cardiac) (TNNT2), transcript variant 2 (as WB positive control)
  • LY424349 is the same product as LY425027.
Size:
100 ug 20 ug
Formulation:
Liquid Lyophilized
  $436.00
5 Days*
Bulk/Customize

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Other products for "Cardiac Troponin T or NM_001001430"

Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC214241
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_001001430, NP_001001430
Synonyms CMD1D; CMH2; CMPD2; cTnT; LVNC6; RCM3; TnTC
Predicted MW 34.4 kDa
Components 1 vial of 100 µg total protein derived from gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
Storage The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 1x SDS-PAGE running buffer (with reducing agent). Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C.
Shipping Dry Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.).
Reference Data
RefSeq NP_001001430
Locus ID 7139
Cytogenetics 1q32.1
Protein Families WB
Protein Pathways Cardiac muscle contraction, Dilated cardiomyopathy, Hypertrophic cardiomyopathy (HCM)
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Other "Cardiac Troponin T" proteins (16)
SKU Description Size Price
PH301218 TNNT2 MS Standard C13 and N15-labeled recombinant protein (NP_001001431) 10 ug
$3,360.00
PH314180 TNNT2 MS Standard C13 and N15-labeled recombinant protein (NP_000355) 10 ug
$3,360.00
PH314241 TNNT2 MS Standard C13 and N15-labeled recombinant protein (NP_001001430) 10 ug
$3,360.00
PH314299 TNNT2 MS Standard C13 and N15-labeled recombinant protein (NP_001001432) 10 ug
$3,360.00
LC400130 Lyophilized TNNT2 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LC424349 Lyophilized TNNT2 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LC424350 Lyophilized TNNT2 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LC424351 Lyophilized TNNT2 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LY400130 Transient overexpression lysate of troponin T type 2 (cardiac) (TNNT2), transcript variant 1 (as WB positive control) 100 ug
$436.00
LY424350 Transient overexpression lysate of troponin T type 2 (cardiac) (TNNT2), transcript variant 3 (as WB positive control) 100 ug
$436.00
LY424351 Transient overexpression lysate of troponin T type 2 (cardiac) (TNNT2), transcript variant 4 (as WB positive control) 100 ug
$436.00
TP301218 Recombinant protein of human troponin T type 2 (cardiac) (TNNT2), transcript variant 3, 20 µg 20 ug
$867.00
TP314180 Recombinant protein of human troponin T type 2 (cardiac) (TNNT2), transcript variant 1, 20 µg 20 ug
$867.00
TP314241 Purified recombinant protein of Homo sapiens troponin T type 2 (cardiac) (TNNT2), transcript variant 2, 20 µg 20 ug
$867.00
TP314299 Purified recombinant protein of Homo sapiens troponin T type 2 (cardiac) (TNNT2), transcript variant 4, 20 µg 20 ug
$867.00
TP750172 Purified recombinant protein of Human troponin T type 2 (cardiac) (TNNT2), transcript variant 1, full length, with N-terminal HIS tag, expressed in E.Coli, 50 ug 50 ug
$270.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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