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LTBP4 (NM_001042544) Human Over-expression Lysate

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SKU
LY420974
Transient overexpression lysate of latent transforming growth factor beta binding protein 4 (LTBP4), transcript variant 1 (as WB positive control)
  $665.00
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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC224230
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_001042544, NP_001036009
Synonyms ARCL1C; LTBP-4; LTBP4L; LTBP4S
Predicted MW 173.3 kDa
Components 1 vial of 100 µg total protein derived from gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
Storage The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 1x SDS-PAGE running buffer (with reducing agent). Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C.
Shipping Dry Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.).
Reference Data
RefSeq NP_001036009
Locus ID 8425
Cytogenetics 19q13.2
Protein Families WB
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Documents
Resources
Other "LTBP4" proteins (1)
SKU Description Size Price
LC420974 Lyophilized LTBP4 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00

file-alt Citations

Overexpression of Latent TGFβ Binding Protein 4 in Muscle Ameliorates Muscular Dystrophy through Myostatin and TGFβ
Lamar, Bogdanovich, Gardner et al
PLoS Genet. (2016) 12 (5), e1006019 DOI: 10.1371/journal.pgen.1006019
Cited by 47
AI Summary: OriGene products contributed to this study by providing cDNA clones for Ltbp4 (MR227629), Gdf11 (MR223819), Tgfβ1 (MR227339), Tgfβ2 (MR225633), and Tgfβ3 (MR206441), which were used to investigate the interactions and roles of these genes in muscle physiology and muscular dystrophy.
Snippet: > (NM_010834), Gdf11 (NM_010272), Tgfβ1 (NM_011577), Tgfβ2 (NM_009367), and Tgfβ3 (NM_009368) were purchased from Origene (catalog numbers MR227629, MR223819, MR227339, MR225633, and MR206441, respectively). A myc
FigureFigureFigure
Genotype-Specific Interaction of Latent TGFβ Binding Protein 4 with TGFβ
Lamar, Miller, Dellefave-Castillo et al
PLoS ONE (2016) 11 (2), e0150358 DOI: 10.1371/journal.pone.0150358
Cited by 19
AI Summary: OriGene products contributed full-length human LTBP4 (catalog #SC311430) and TGFβ1 (catalog #SC119746) cDNA clones, which were used to study genotype-specific interactions between LTBP4 and TGFβ in this research.
Snippet: Full length human LTBP4 (NM_001042544.1) and TGFβ1 (NM_000660.3) cDNA clones were purchased from Origene (catalog numbers SC311430 and SC119746, respectively). TGFβ2 (NM_003238.3) and TGFβ3 (NM_003239.2) were amplified by polymerase chain reaction using cDNA from human skin fibroblasts (GM03348, Coriell Cell Repositories) as a template. …The Xpress epitope (DLYDDDDK) was added onto the 5’/N-terminus of LTBP4
FigureFigureFigure
Showing 1-2 of 2 papers.
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