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AMFR (NM_001144) Human Over-expression Lysate

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SKU
LY420107
Transient overexpression lysate of autocrine motility factor receptor (AMFR) (as WB positive control)
  $436.00
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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC209639
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_001144, NP_001135
Synonyms GP78; RNF45
Predicted MW 73 kDa
Components 1 vial of 100 µg total protein derived from gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
Storage The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 1x SDS-PAGE running buffer (with reducing agent). Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C.
Shipping Dry Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.).
Reference Data
RefSeq NP_001135
Locus ID 267
Cytogenetics 16q13
Protein Families WB
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Other "AMFR" proteins (1)
SKU Description Size Price
LC420107 Lyophilized AMFR HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00

file-alt Citations

Affinity proteomics discovers decreased levels of AMFR in plasma from Osteoporosis patients
Qundos, Drobin, Mattsson et al
Proteomics Clin Appl (2016) 10 (6), 681-90 DOI: 10.1002/prca.201400167
Cited by 17
AI Summary: OriGene’s AMFR overexpressing cell lysate (LY420107) was used as a positive control in Western blot experiments to validate antibody specificity in the detection of AMFR protein levels in plasma samples.
Snippet: A second Western blot protocol was performed with a plasma sample from a healthy donor pool depleted from human serum albumin and immunoglobulin G using Affibody molecules (Affibody AB) 16, lysate from AMFR overexpressing cells (LY420107, Origene) and provided negative control cells (lacking AMFR vector)
FigureFigureFigure
Affinity proteomics discovers decreased levels of AMFR in plasma from Osteoporosis patients
Qundos, Drobin, Mattsson et al
Proteomics Clin Appl (2015)
Cited by 17
AI Summary: In this study, OriGene's AMFR over-expressing cell lysate (LY420107) was used as a positive control in Western blot experiments to verify the selectivity of the anti-AMFR antibody, supporting the identification of decreased AMFR levels in the plasma of osteoporosis patients.
Snippet: to this, a second Western blot protocol used a plasma sample from a healthy donor pool was depleted from human serum albumin and immunoglobulin G using Affibody molecules (Affibody AB) [16], lysate from AMFR over- expressing cells (LY420107, Origene) and ... Cite SaveSaving
Showing 1-2 of 2 papers.
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