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HDAC2 (NM_001527) Human Over-expression Lysate

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SKU
LY419878
Transient overexpression lysate of histone deacetylase 2 (HDAC2) (as WB positive control)
  $665.00
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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC224919
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_001527, NP_001518
Synonyms HD2; KDAC2; RPD3; YAF1
Predicted MW 66 kDa
Components 1 vial of 100 µg total protein derived from gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
Storage The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 1x SDS-PAGE running buffer (with reducing agent). Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C.
Shipping Dry Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.).
Reference Data
RefSeq NP_001518
Locus ID 3066
Cytogenetics 6q21
Protein Families WB
Protein Pathways Cell cycle, Chronic myeloid leukemia, Huntington's disease, Notch signaling pathway, Pathways in cancer
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Other "HDAC2" proteins (2)
SKU Description Size Price
LC419878 Lyophilized HDAC2 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
TP762594 Purified recombinant protein of Human histone deacetylase 2 (HDAC2), transcript variant 1, full length, with N-terminal GST and C-terminal His tag, expressed in E.coli, 50ug 50 ug
$270.00

file-alt Citations

Chemopreventive effects of an HDAC2-selective inhibitor on rat colon carcinogenesis and APCmin/+ mouse intestinal tumorigenesis
Ravillah, Mohammed, Li et al
J. Pharmacol. Exp. Ther. (2013)
Cited by 14
AI Summary: OriGene Technologies contributed to this study by providing the primary antibody to HDAC2, which was used to assess changes in HDAC2 expression levels in response to chemopreventive treatments.
Snippet: procured from the Division of Cancer Prevention, National Cancer Institute Chemoprevention drug repository (Rockville, MD). Primary antibody to HDAC2 was from OriGene Technologies (Rockville, MD);anti-CDC25C, anti-CDK2, anti-PCNA, anti-Bcl2 and anti-p53 ... Cite SaveSaving
Showing 1-1 of 1 papers.
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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