SLC25A6 (NM_001636) Human Over-expression Lysate
SKU
LY419829
Transient overexpression lysate of solute carrier family 25 (mitochondrial carrier; adenine nucleotide translocator), member 6 (SLC25A6), nuclear gene encoding mitochondrial protein (as WB positive control)
$436.00
In Stock*
Specifications
| Product Data | |
| Species | Human |
|---|---|
| Expression Host | HEK293T |
| Expression cDNA Clone or AA Sequence | TrueORF Clone RC203878 |
| Tag | C-Myc/DDK |
| Detection Antibodies | Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100) |
| ACCN | NM_001636, NP_001627 |
| Synonyms | AAC3; ANT; ANT 2; ANT 3; ANT3; ANT3Y |
| Predicted MW | 32.9 kDa |
| Components | 1 vial of 100 µg total protein derived from gene specific transient over-expression cell lysate in RIPA buffer 1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer |
| Storage | The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 1x SDS-PAGE running buffer (with reducing agent). Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. |
| Shipping | Dry Ice |
| Preparation | HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). |
| Reference Data | |
| RefSeq | NP_001627 |
| Locus ID | 293 |
| Cytogenetics | X;Y |
| Protein Families | WB |
| Protein Pathways | Calcium signaling pathway, Huntington's disease, Parkinson's disease |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
| Recombinant Protein Resources |
Other "SLC25A6" proteins (1)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| LC419829 | Lyophilized SLC25A6 HEK293T cell transient overexpression lysate (as WB positive control) | 20 ug |
$206.00
|
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Citations
TIM29 is a subunit of the human carrier translocase required for protein transport
Callegari, Richter, Chojnacka et al
FEBS Lett. (2016) 590 (23), 4147-4158 DOI: 10.1002/1873-3468.12450
Callegari, Richter, Chojnacka et al
FEBS Lett. (2016) 590 (23), 4147-4158 DOI: 10.1002/1873-3468.12450
AI Summary: OriGene’s pCMV-Entry-SLC25A6 plasmid was used as the template to amplify ANT3 by PCR, enabling in vitro synthesis of labeled precursor proteins for studying protein import by the TIM22 complex.
Snippet: ANT3 was amplified by PCR from pCMV‐Entry‐SLC25A6 (OriGene, Rockville, MD, USA) using primers containing the SP6 promoter site. [35S]‐labeled precursor proteins were synthesized in vitro with the TNT SP6 Quick Coupled Transcription/Translation system (Promega, Madison, WI, USA) using the respective RNA
Showing 1-1 of 1 papers.
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complexities in the preparation of your product. International customers may expect an additional 1-2 weeks
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