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CRABP2 (NM_001878) Human Over-expression Lysate

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SKU
LY419677
Transient overexpression lysate of cellular retinoic acid binding protein 2 (CRABP2) (as WB positive control)
  $436.00
5 Days*
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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC200221
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_001878, NP_001869
Synonyms CRABP-II; RBP6
Predicted MW 15.7 kDa
Components 1 vial of 100 µg total protein derived from gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
Storage The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 1x SDS-PAGE running buffer (with reducing agent). Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C.
Shipping Dry Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.).
Reference Data
RefSeq NP_001869
Locus ID 1382
Cytogenetics 1q23.1
Protein Families WB
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Other "CRABP2" proteins (3)
SKU Description Size Price
PH300221 CRABP2 MS Standard C13 and N15-labeled recombinant protein (NP_001869) 10 ug
$3,360.00
LC419677 Lyophilized CRABP2 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
TP300221 Recombinant protein of human cellular retinoic acid binding protein 2 (CRABP2), 20 µg 20 ug
$867.00

file-alt Citations

Dissecting antibodies with regards to linear and conformational epitopes
Forsström, Bisławska Axnäs, Rockberg et al
PLoS ONE (2015) 10 (3), e0121673 DOI: 10.1371/journal.pone.0121673
Cited by 130
AI Summary: OriGene’s CRABP2 overexpression lysate (LY419677) was used as a source of target protein to assess antibody binding and specificity in Western blot assays in this study.
Snippet: on Western blot membranes prepared from 15 μg of total protein lysates from one or more of the three cell lines (A431, RT-4 and U-251 MG), two tissues (liver and tonsil), plasma and HEK293 cells over-expressing the target protein CRABP2 (LY419677, Origene Technologies ... Cite SaveSaving
Showing 1-1 of 1 papers.
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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