OriGene Logo

Customer Login

Checkout as a new customer

Creating an account has many benefits:

  • See order and shipping status
  • Track order history
  • Check out faster
Create an Account

CCN2 (NM_001901) Human Over-expression Lysate

Be the first to review this product
SKU
LY419668
Transient overexpression lysate of connective tissue growth factor (CTGF) (as WB positive control)
  $436.00
2 Weeks*
Bulk/Customize

Frequently Bought Together (3)

TA806803 CTGF mouse monoclonal antibody, clone OTI5H7 (formerly 5H7) 100 ul
$447.00
TA811000S beta Actin Mouse Monoclonal Antibody, Clone OTI1, Loading Control 30 ul
$200.00
TA592569 DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
100 ul
$519.00

Other products for "CCN2 or NM_001901"

Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC210336
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_001901, NP_001892
Synonyms CTGF; HCS24; IGFBP8; NOV2
Predicted MW 38.1 kDa
Components 1 vial of 100 µg total protein derived from gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
Storage The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 1x SDS-PAGE running buffer (with reducing agent). Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C.
Shipping Dry Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.).
Reference Data
RefSeq NP_001892
Locus ID 1490
Cytogenetics 6q23.2
Protein Families WB
Reviews
Write Review
 
 
 
 
 

Be the first to review this product

Please note:
Only reviews with images are eligible for a $20/20€ Amazon gift card.
Reviews without images are eligible for a $10/10€ Amazon gift card.
For more details about the terms and conditions, please visit the promotion page.

Review image
Documents
Resources
Other "CCN2" proteins (2)
SKU Description Size Price
LC419668 Lyophilized CTGF HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
TP710182 Recombinant protein of human connective tissue growth factor (CTGF), residues 27-349aa, secretory expressed with GP67 signal peptide, with C-terminal DDK tag, expressed in sf9, 20ug 20 ug
$540.00

file-alt Citations

ALCAM is indirectly modulated by miR-125b in MCF7 cells
Akman, Selcuklu, Donoghue et al
Tumour Biol. (2014)
Cited by 5
Snippet: One-way ANOVA with Tukey's multiple comparison post test was performed using GraphPad Prism software. For patient samples, Origene Breast Cancer cDNA array IV (BCRT504) was used (three technical replicates) (for patient characteristics, please see Supp. ... Cite SaveSaving
Showing 1-1 of 1 papers.
Powered by BizGenius
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Related Products