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Desmoglein 2 (DSG2) (NM_001943) Human Over-expression Lysate

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SKU
LY419645
Transient overexpression lysate of desmoglein 2 (DSG2) (as WB positive control)
  $436.00
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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC212294
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_001943, NP_001934
Synonyms CDHF5; HDGC
Predicted MW 122.29 kDa
Components 1 vial of 100 µg total protein derived from gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
Storage The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 1x SDS-PAGE running buffer (with reducing agent). Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C.
Shipping Dry Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.).
Reference Data
RefSeq NP_001934
Locus ID 1829
Cytogenetics 18q12.1
Protein Families WB
Protein Pathways Arrhythmogenic right ventricular cardiomyopathy (ARVC)
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Other "Desmoglein 2" proteins (1)
SKU Description Size Price
LC419645 Lyophilized DSG2 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00

file-alt Citations

Desmoglein-2 expression is an independent predictor of poor prognosis patients with multiple myeloma
Ebert, Vandyke, Johan et al
Mol Oncol (2022) 16 (6), 1221-1240 DOI: 10.1002/1878-0261.13055
Cited by 17
AI Summary: In this study, OriGene products including the DSG2 cDNA clone (OriGene, RC211232) and DSG2 shRNA lentiviral particles (OriGene, TL313590V) were used to manipulate DSG2 expression in multiple myeloma cell lines, enabling the authors to investigate the functional role of DSG2 in cell adhesion and its association with poor prognosis in patients.
FigureFigureFigure
Showing 1-1 of 1 papers.
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