GARS1 (NM_002047) Human Over-expression Lysate
SKU
LY419568
Transient overexpression lysate of glycyl-tRNA synthetase (GARS) (as WB positive control)
$436.00
5 Days*
Specifications
| Product Data | |
| Species | Human |
|---|---|
| Expression Host | HEK293T |
| Expression cDNA Clone or AA Sequence | TrueORF Clone RC202852 |
| Tag | C-Myc/DDK |
| Detection Antibodies | Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100) |
| ACCN | NM_002047, NP_002038 |
| Synonyms | CMT2D; DSMAV; GARS; GlyRS; HMN5; HMN5A; SMAD1; SMAJI |
| Predicted MW | 83.1 kDa |
| Components | 1 vial of 100 µg total protein derived from gene specific transient over-expression cell lysate in RIPA buffer 1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer |
| Storage | The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 1x SDS-PAGE running buffer (with reducing agent). Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. |
| Shipping | Dry Ice |
| Preparation | HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). |
| Reference Data | |
| RefSeq | NP_002038 |
| Locus ID | 2617 |
| Cytogenetics | 7p14.3 |
| Protein Families | WB |
| Protein Pathways | Aminoacyl-tRNA biosynthesis |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
| Recombinant Protein Resources |
Other "GARS1" proteins (3)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| PH302852 | GARS MS Standard C13 and N15-labeled recombinant protein (NP_002038) | 10 ug |
$3,360.00
|
|
| LC419568 | Lyophilized GARS HEK293T cell transient overexpression lysate (as WB positive control) | 20 ug |
$206.00
|
|
| TP302852 | Recombinant protein of human glycyl-tRNA synthetase (GARS), 20 µg | 20 ug |
$737.00
|
|
Citations
HDAC6 is a therapeutic target in mutant GARS-induced Charcot-Marie-Tooth disease
Benoy, Van Helleputte, Prior et al
Brain (2018)
Benoy, Van Helleputte, Prior et al
Brain (2018)
AI Summary: OriGene’s GlyRS-targeting siRNA kit (SR419603) was used in this study to specifically reduce GlyRS expression and investigate its role in Charcot-Marie-Tooth disease mechanisms.
Snippet: according to manufacturer’s instructions. Small interfering RNA technology, comprising three different siRNA molecules and one control siRNA molecule, was used to target GlyRS expression (SR419603, Origene). Bioinformatic tools (Ryan _et al._, 2008;and BLAST – US National Library of Medicine) were used to check specificity of the siRNAs towards mouse _Gars_ mRNA. Plasmids containing short hai
Showing 1-1 of 1 papers.
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen
complexities in the preparation of your product. International customers may expect an additional 1-2 weeks
in shipping.
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