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PDE8A (NM_002605) Human Over-expression Lysate

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SKU
LY419223
Transient overexpression lysate of phosphodiesterase 8A (PDE8A), transcript variant 1 (as WB positive control)
  $436.00
5 Days*
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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC209169
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_002605, NP_002596
Synonyms HsT19550
Predicted MW 93.2 kDa
Components 1 vial of 100 µg total protein derived from gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
Storage The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 1x SDS-PAGE running buffer (with reducing agent). Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C.
Shipping Dry Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.).
Reference Data
RefSeq NP_002596
Locus ID 5151
Cytogenetics 15q25.3
Protein Families WB
Protein Pathways Progesterone-mediated oocyte maturation, Purine metabolism
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Documents
Resources
Other "PDE8A" proteins (1)
SKU Description Size Price
LC419223 Lyophilized PDE8A HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00

file-alt Citations

Phosphodiesterase 8a Supports HIV-1 Replication in Macrophages at the Level of Reverse Transcription
Booiman, Cobos Jiménez, van Dort et al
PLoS ONE (2014) 9 (10), e109673 DOI: 10.1371/journal.pone.0109673
Cited by 6
AI Summary: OriGene’s MYC-tagged PDE8A expression vector was used in this study to overexpress PDE8A and test shRNA knockdown efficiency, enabling the authors to investigate the role of PDE8A in supporting HIV-1 replication in macrophages.
Snippet: Plasmids. For PDE8A overexpression and testing knockdown efficiency of the shRNAs targeting PDE8A, a vector expressing a MYC-tagged PDE8A under the control of the CMV promotor (Origene, Rockville, MD, USA) was used. ... Cite SaveSaving
Showing 1-1 of 1 papers.
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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