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Serum Response Factor (SRF) (NM_003131) Human Over-expression Lysate

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SKU
LY418874
Transient overexpression lysate of serum response factor (c-fos serum response element-binding transcription factor) (SRF) (as WB positive control)
  $436.00
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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC208596
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_003131, NP_003122
Synonyms MCM1
Predicted MW 51.4 kDa
Components 1 vial of 100 µg total protein derived from gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
Storage The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 1x SDS-PAGE running buffer (with reducing agent). Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C.
Shipping Dry Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.).
Reference Data
RefSeq NP_003122
Locus ID 6722
Cytogenetics 6p21.1
Protein Families WB
Protein Pathways MAPK signaling pathway
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Other "Serum Response Factor" proteins (3)
SKU Description Size Price
PH308596 SRF MS Standard C13 and N15-labeled recombinant protein (NP_003122) 10 ug
$3,360.00
LC418874 Lyophilized SRF HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
TP308596 Recombinant protein of human serum response factor (c-fos serum response element-binding transcription factor) (SRF), 20 µg 20 ug
$867.00

file-alt Citations

Interactions of Antibodies to the Gram-Negative Gastric Bacterium Helicobacter pylori with the Synaptic Calcium Sensor Synaptotagmin 5, Correlate to Impaired Vesicle Recycling in SiMa Human Neuroblastoma Cells
Kleine, Reuss
J. Mol. Neurosci. (2020)
Cited by 4
AI Summary: OriGene’s Untransfected HEK293 control lysate (cat. no. LY500001) and Human Syt5-transfected HEK293 overexpression lysate (cat. no. LY418847) were used in this study to confirm antibody interactions with synaptotagmin 5 by Western blot analysis.
Snippet: …LY500001). Human Syt5-transfected HEK293 overexpression lysate (Origene, LY418847);- no. 31450). …LY500001). Human Syt5-transfected HEK293 overexpression lysate (Origene, LY418847)
Showing 1-1 of 1 papers.
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