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Aurora A (AURKA) (NM_003600) Human Over-expression Lysate

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SKU
LY418569
Transient overexpression lysate of aurora kinase A (AURKA), transcript variant 2 (as WB positive control)
  • LY418569 is the same product as LY429157.
Size:
100 ug 20 ug
Formulation:
Liquid Lyophilized
  $436.00
5 Days*
Bulk/Customize

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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC216460
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_003600, NP_003591
Synonyms AIK; ARK1; AURA; BTAK; PPP1R47; STK6; STK7; STK15
Predicted MW 45.6 kDa
Components 1 vial of 100 µg total protein derived from gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
Storage The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 1x SDS-PAGE running buffer (with reducing agent). Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C.
Shipping Dry Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.).
Reference Data
RefSeq NP_003591
Locus ID 6790
Cytogenetics 20q13.2
Protein Families WB
Protein Pathways Oocyte meiosis
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Other "Aurora A" proteins (20)
SKU Description Size Price
PH302904 AURKA MS Standard C13 and N15-labeled recombinant protein (NP_940839) 10 ug
$3,360.00
PH306572 AURKA MS Standard C13 and N15-labeled recombinant protein (NP_940838) 10 ug
$3,360.00
PH312018 AURKA MS Standard C13 and N15-labeled recombinant protein (NP_940835) 10 ug
$3,360.00
PH316513 AURKA MS Standard C13 and N15-labeled recombinant protein (NP_940836) 10 ug
$3,360.00
LC403678 Lyophilized AURKA HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LC404924 Lyophilized AURKA HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LC404925 Lyophilized AURKA HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LC404926 Lyophilized AURKA HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LC404927 Lyophilized AURKA HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LC418569 Lyophilized AURKA HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LY403678 Transient overexpression lysate of aurora kinase A (AURKA), transcript variant 1 (as WB positive control) 100 ug
$436.00
LY404924 Transient overexpression lysate of aurora kinase A (AURKA), transcript variant 3 (as WB positive control) 100 ug
$436.00
LY404925 Transient overexpression lysate of aurora kinase A (AURKA), transcript variant 4 (as WB positive control) 100 ug
$436.00
LY404926 Transient overexpression lysate of aurora kinase A (AURKA), transcript variant 5 (as WB positive control) 100 ug
$436.00
LY404927 Transient overexpression lysate of aurora kinase A (AURKA), transcript variant 6 (as WB positive control) 100 ug
$436.00
TP302904 Recombinant protein of human aurora kinase A (AURKA), transcript variant 6, 20 µg 20 ug
$867.00
TP306572 Recombinant protein of human aurora kinase A (AURKA), transcript variant 5, 20 µg 20 ug
$867.00
TP312018 Recombinant protein of human aurora kinase A (AURKA), transcript variant 1, 20 µg 20 ug
$867.00
TP316513 Recombinant protein of human aurora kinase A (AURKA), transcript variant 3, 20 µg 20 ug
$867.00
TP762392 Purified recombinant protein of Human aurora kinase A (AURKA), transcript variant 4, Met1-Met300, with N-terminal His tag, expressed in E.coli, 50ug 50 ug
$260.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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