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PPM1D (NM_003620) Human Over-expression Lysate

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SKU
LY418539
Transient overexpression lysate of protein phosphatase 1D magnesium-dependent, delta isoform (PPM1D) (as WB positive control)
  $436.00
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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC209328
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_003620, NP_003611
Synonyms IDDGIP; JDVS; PP2C-DELTA; WIP1
Predicted MW 66.5 kDa
Components 1 vial of 100 µg total protein derived from gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
Storage The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 1x SDS-PAGE running buffer (with reducing agent). Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C.
Shipping Dry Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.).
Reference Data
RefSeq NP_003611
Locus ID 8493
Cytogenetics 17q23.2
Protein Families WB
Protein Pathways p53 signaling pathway
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Other "PPM1D" proteins (3)
SKU Description Size Price
PH309328 PPM1D MS Standard C13 and N15-labeled recombinant protein (NP_003611) 10 ug
$3,360.00
LC418539 Lyophilized PPM1D HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
TP309328 Purified recombinant protein of Homo sapiens protein phosphatase 1D magnesium-dependent, delta isoform (PPM1D), 20 µg 20 ug
$737.00

file-alt Citations

Recurrent initiation: a mechanism for triggering p53 pulses in response to DNA damage
Batchelor, Mock, Bhan et al
Mol Cell (2008) 30 (3), 277-89 DOI: 10.1016/j.molcel.2008.03.016
Cited by 438
AI Summary: In this study, OriGene products—specifically, the p53 (TP53) cDNA clone and Chk2 (CHEK2) shRNA lentiviral particles—were used to manipulate gene expression and investigate the roles of p53 and Chk2 in the generation and regulation of p53 pulses following DNA damage.
FigureFigure
Showing 1-1 of 1 papers.
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