ITGA8 (NM_003638) Human Over-expression Lysate

CAT#: LY418532

Transient overexpression lysate of integrin, alpha 8 (ITGA8)

Bulk Request/Custom Protein Development

USD 495.00


Availability*
5 Days

Size
    • 100 ug

Product images

Specifications

Product Data
Description Transient overexpression lysate of integrin, alpha 8 (ITGA8)
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC218936
Tag C-MYC/DDK
Detection Antibodies Anti-DDK Antibody (TA50011-100)
Accession Number NM_003638, NP_003629
Other Names ITGA8, OTTHUMP00000019207; integrin, alpha 8
Predicted MW 117.3 kDa
Components 1 vial of 100 µg gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg empty vector transfected control cell lysate in RIPA buffer
1 vial of 250ul 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromphenol blue, 100mM DTT)
Storage The lysate is shipped with dry ice. Upon receiving, store the sample at -20ºC. Lysate samples are stable for 12 months from date of receipt when stored at -20ºC. Avoid repeated freeze-thaw cycles.
Lysate samples can be diluted with 2xSDS Sample Buffer provided. After dilution, the protein sample should be aliquoted and stored at -20ºC for long term storage. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes.
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). Cell lysates were aliquoted and stored at -20ºC before shipping.
Reference Data
RefSeq NP_003629
Locus ID 8516
Cytogenetics OTTHUMP00000019207; integrin, alpha 8
Protein Families Transmembrane, Druggable Genome
Protein Pathways Focal adhesion, ECM-receptor interaction, Cell adhesion molecules (CAMs), Regulation of actin cytoskeleton, Hypertrophic cardiomyopathy (HCM), Arrhythmogenic right ventricular cardiomyopathy (ARVC), Dilated cardiomyopathy
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.