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MED1 (NM_004774) Human Over-expression Lysate

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SKU
LY417755
Transient overexpression lysate of mediator complex subunit 1 (MED1) (as WB positive control)
  $665.00
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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC222118
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_004774, NP_004765
Synonyms CRSP1; CRSP200; DRIP205; DRIP230; PBP; PPARBP; PPARGBP; RB18A; TRAP220; TRIP2
Predicted MW 168.3 kDa
Components 1 vial of 100 µg total protein derived from gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
Storage The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 1x SDS-PAGE running buffer (with reducing agent). Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C.
Shipping Dry Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.).
Reference Data
RefSeq NP_004765
Locus ID 5469
Cytogenetics 17q12
Protein Families WB
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Other "MED1" proteins (1)
SKU Description Size Price
LC417755 Lyophilized MED1 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00

file-alt Citations

Mediator subunit MED1 modulates intranuclear dynamics of the thyroid hormone receptor
Femia, Evans, Zhang et al
J. Cell. Biochem. (2019)
AI Summary: OriGene products, specifically the GFP-MED1 plasmid (encoding full-length human MED1) and the pCMV-MIR208a (miR-208a expression plasmid), were used in this study to manipulate MED1 and miR-208a levels, enabling the authors to investigate their roles in modulating the nuclear retention and dynamics of thyroid hormone receptors.
Snippet: GFP‐MED1 (OriGene Technologies, Inc) encodes full‐length human MED1 (residues 1‐1581) pCMV‐MIR, a scrambled microRNA (miRNA) and GFP expression plasmid;and pCMV‐MIR208a, a miR‐208a and GFP expression plasmid were from Origene Technologies
Showing 1-1 of 1 papers.
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