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LONP1 (NM_004793) Human Over-expression Lysate

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SKU
LY417746
Transient overexpression lysate of lon peptidase 1, mitochondrial (LONP1), nuclear gene encoding mitochondrial protein (as WB positive control)
  $436.00
5 Days*
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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC200359
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_004793, NP_004784
Synonyms CODASS; hLON; LON; LonHS; LONP; PIM1; PRSS15
Predicted MW 106.5 kDa
Components 1 vial of 100 µg total protein derived from gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
Storage The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 1x SDS-PAGE running buffer (with reducing agent). Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C.
Shipping Dry Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.).
Reference Data
RefSeq NP_004784
Locus ID 9361
Cytogenetics 19p13.3
Protein Families WB
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Documents
Resources
Other "LONP1" proteins (1)
SKU Description Size Price
LC417746 Lyophilized LONP1 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00

file-alt Citations

LonP1 as a Key Driver of Tumor Progression and a Therapeutic Target in IDH Mutant Astrocytoma
Douglas
Thesis (2023)
Snippet: Diffuse glioma is designated as grade III-IV and has poor overall survival with inevitable recurrence. The IDH1 mutation co-occurring with 1p/19q codeletion classifies a Save;The total proteolytic activity of purified recombinant LonP1 (Origene, LY417746) was analyzed using a Pierce Fluorescent Protease Assay Kit (Thermo Scientific , 23266). A mixture of 200 nM
Proteomic Analysis of Mitochondrial-Associated ER Membranes (MAM) during RNA Virus Infection Reveals Dynamic Changes in Protein and Organelle Trafficking
Horner, Wilkins, Badil et al
PLoS ONE (2015) 10 (3), e0117963 DOI: 10.1371/journal.pone.0117963
Cited by 126
AI Summary: OriGene products contributed plasmids encoding human VTN (NM_000638.3), LONP1 (NM_004793.2), and RAB1B (NM_030981.1), which were used to investigate the roles of these proteins in MAVS signalosome assembly and innate immune signaling during RNA virus infection.
Snippet: Laboratory. Plasmids and transfections. The pMyc-Mavs [20] plasmid has been described previously. Plasmids encoding human VTN (NM_000638.3), LONP1 (NM_004793.2), and RAB1B (NM_030981.1) were obtained (Origene). ... Cite SaveSaving
Showing 1-2 of 2 papers.
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