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alpha Actinin 4 (ACTN4) (NM_004924) Human Over-expression Lysate

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SKU
LY417652
Transient overexpression lysate of actinin, alpha 4 (ACTN4) (as WB positive control)
  $436.00
5 Days*
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Other products for "alpha Actinin 4 or NM_004924"

Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC202873
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_004924, NP_004915
Synonyms ACTININ-4; FSGS; FSGS1
Predicted MW 104.9 kDa
Components 1 vial of 100 µg total protein derived from gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
Storage The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 1x SDS-PAGE running buffer (with reducing agent). Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C.
Shipping Dry Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.).
Reference Data
RefSeq NP_004915
Locus ID 81
Cytogenetics 19q13.2
Protein Families WB
Protein Pathways Adherens junction, Arrhythmogenic right ventricular cardiomyopathy (ARVC), Focal adhesion, Leukocyte transendothelial migration, Regulation of actin cytoskeleton, Systemic lupus erythematosus, Tight junction
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Other "alpha Actinin 4" proteins (3)
SKU Description Size Price
PH302873 ACTN4 MS Standard C13 and N15-labeled recombinant protein (NP_004915) 10 ug
$3,360.00
LC417652 Lyophilized ACTN4 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
TP302873 Recombinant protein of human actinin, alpha 4 (ACTN4), 20 µg 20 ug
$737.00

file-alt Citations

Alpha-actinin 4 is associated with cancer cell motility and is a potential biomarker in non-small cell lung cancer
Wang, Chang, Wu et al
J Thorac Oncol (2015) 10 (2), 286-301 DOI: 10.1097/JTO.0000000000000396
AI Summary: OriGene products contributed to this study by providing the ACTN4 shRNA (TG311513) used for RNA interference-mediated knockdown of ACTN4, enabling the authors to investigate the functional role of ACTN4 in non-small cell lung cancer cell motility and invasion.
Showing 1-1 of 1 papers.
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