OriGene Logo

Customer Login

Checkout as a new customer

Creating an account has many benefits:

  • See order and shipping status
  • Track order history
  • Check out faster
Create an Account

ROD1 (PTBP3) (NM_005156) Human Over-expression Lysate

Be the first to review this product
SKU
LY417476
Transient overexpression lysate of ROD1 regulator of differentiation 1 (S. pombe) (ROD1), transcript variant 1 (as WB positive control)
  $665.00
In Stock*
Bulk/Customize

Frequently Bought Together (3)

TA811000S beta Actin Mouse Monoclonal Antibody, Clone OTI1, Loading Control 30 ul
$200.00
TA812031 PTBP3 mouse monoclonal antibody,clone OTI5G7 100 ul
$447.00
TA592569 DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
100 ul
$519.00

Other products for "ROD1 or NM_005156"

Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC211812
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_005156, NP_005147
Synonyms ROD1
Predicted MW 59.5 kDa
Components 1 vial of 100 µg total protein derived from gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
Storage The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 1x SDS-PAGE running buffer (with reducing agent). Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C.
Shipping Dry Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.).
Reference Data
RefSeq NP_005147
Locus ID 9991
Cytogenetics 9q32
Protein Families WB
Reviews
Write Review
 
 
 
 
 

Be the first to review this product

Please note:
Only reviews with images are eligible for a $20/20€ Amazon gift card.
Reviews without images are eligible for a $10/10€ Amazon gift card.
For more details about the terms and conditions, please visit the promotion page.

Review image
Documents
Resources
Other "ROD1" proteins (5)
SKU Description Size Price
LC417476 Lyophilized PTBP3 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LC431455 Lyophilized PTBP3 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LC431475 Lyophilized PTBP3 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LY431455 Transient overexpression lysate of ROD1 regulator of differentiation 1 (S. pombe) (ROD1), transcript variant 2 (as WB positive control) 100 ug
$436.00
LY431475 Transient overexpression lysate of ROD1 regulator of differentiation 1 (S. pombe) (ROD1), transcript variant 3 (as WB positive control) 100 ug
$436.00

file-alt Citations

ROD1 is a seedless target gene of hypoxia-induced miR-210
Fasanaro, Romani, Voellenkle et al
PLoS ONE (2012) 7 (9), e44651 DOI: 10.1371/journal.pone.0044651
Cited by 41
AI Summary: OriGene’s pCMV6-XL5-ROD1 plasmid (clone number NM_005156.3) was used to over-express ROD1 in cells, enabling the authors to investigate ROD1’s regulation by miR-210 in their study.
Snippet: ROD1 over-expression was obtained by transfection of pCMV6-XL5-ROD1 (clone number NM_005156.3, OriGene) using Fugene6 (Roche). ... tool (Bielefeld University Bioinformatics Server) was used to calculate the minimum free energy hybridization for short RNA duplexes.
FigureFigureFigure
Showing 1-1 of 1 papers.
Powered by BizGenius
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Related Products