Hormone sensitive lipase (LIPE) (NM_005357) Human Over-expression Lysate
SKU
LY417354
Transient overexpression lysate of lipase, hormone-sensitive (LIPE) (as WB positive control)
$436.00
In Stock*
Specifications
| Product Data | |
| Species | Human |
|---|---|
| Expression Host | HEK293T |
| Expression cDNA Clone or AA Sequence | TrueORF Clone RC209729 |
| Tag | C-Myc/DDK |
| Detection Antibodies | Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100) |
| ACCN | NM_005357, NP_005348 |
| Synonyms | AOMS4; FPLD6; HSL; LHS; REH |
| Predicted MW | 116.6 kDa |
| Components | 1 vial of 100 µg total protein derived from gene specific transient over-expression cell lysate in RIPA buffer 1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer |
| Storage | The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 1x SDS-PAGE running buffer (with reducing agent). Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. |
| Shipping | Dry Ice |
| Preparation | HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). |
| Reference Data | |
| RefSeq | NP_005348 |
| Locus ID | 3991 |
| Cytogenetics | 19q13.2 |
| Protein Families | WB |
| Protein Pathways | Insulin signaling pathway |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
| Recombinant Protein Resources |
Other "Hormone sensitive lipase" proteins (3)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| PH309729 | LIPE MS Standard C13 and N15-labeled recombinant protein (NP_005348) | 10 ug |
$3,360.00
|
|
| LC417354 | Lyophilized LIPE HEK293T cell transient overexpression lysate (as WB positive control) | 20 ug |
$206.00
|
|
| TP309729 | Recombinant protein of human lipase, hormone-sensitive (LIPE), 20 µg | 20 ug |
$737.00
|
|
Citations
TLR4 and prostaglandin pathways at the crossroads of endotoxemia-induced lipolysis
Chirivi, Abou-Rjeileh, Myers et al
Front Immunol (2025) 16, 1591210 DOI: 10.3389/fimmu.2025.1591210
Chirivi, Abou-Rjeileh, Myers et al
Front Immunol (2025) 16, 1591210 DOI: 10.3389/fimmu.2025.1591210
Cited by 8
AI Summary: OriGene products contributed to this study by providing over-expression lysates for HSL and ERK, which were used as positive controls in protein expression analyses.
Snippet: 1, ProteinSimple). The normalized data are expressed as relative to CON value, and ratios pHSL(Ser563):HSL and pERK: ERK. Positive controls were over-expression lysates for HSL, and ERK obtained from OriGene Technologies (Rockvilly, MD) while COX-2, PTGER2, and PTGER4 were from Abnova Corporation (Taipei City, Taiwan). 2.8Lipidomics Plasma and cell culture media samples were collected, extracted,
Lipolysis inhibition as a treatment of clinical ketosis in dairy cows: Effects on adipose tissue metabolic and immune responses
Chirivi, Cortes, Rendon et al
J Dairy Sci (2024)
Chirivi, Cortes, Rendon et al
J Dairy Sci (2024)
Cited by 16
AI Summary: OriGene products contributed to this study by providing overexpression lysates for Akt (LC401580) and HSL (LC417354), which were used as positive controls to validate protein detection in adipose tissue samples from dairy cows with clinical ketosis.
Snippet: Dairy cows with clinical ketosis (CK) exhibit excessive adipose tissue (AT) lipolysis and systemic inflammation. Lipolysis in cows can be induced by the canonical (;To validate protein detection, overexpression lysates were used as positive controls for Akt (LC401580, OriGene Technologies, Rockville, MD, USA), HSL (LC417354, OriGene Technologies), and ERK1/2 (042–488, ProteinSimple)
TLR4 Signaling Modulates Lipolysis in Dairy Cows' Adipose Tissues
Chirivi
Thesis (2023)
Chirivi
Thesis (2023)
Snippet: Excessive lipolysis from adipose tissue (AT) is considered a major risk factor of postpartum metabolic disease in dairy cows. Among those, clinical ketosis occurs when Save;To validate protein detection, over-expression lysates were used as positive controls for Akt (LC401580, OriGene Technologies, Rockville, MD, USA), HSL (LC417354, OriGene Technologies), and ERK1/2 (042-488 ProteinSimple)
Showing 1-3 of 3 papers.
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