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IL12RB1 (NM_005535) Human Over-expression Lysate

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SKU
LY417240
Transient overexpression lysate of interleukin 12 receptor, beta 1 (IL12RB1), transcript variant 1 (as WB positive control)
  $665.00
5 Days*
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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC221974
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_005535, NP_005526
Synonyms CD212; IL-12R-BETA1; IL12RB; IMD30
Predicted MW 73.14 kDa
Components 1 vial of 100 µg total protein derived from gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
Storage The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 1x SDS-PAGE running buffer (with reducing agent). Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C.
Shipping Dry Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.).
Reference Data
RefSeq NP_005526
Locus ID 3594
Cytogenetics 19p13.11
Protein Families WB
Protein Pathways Cytokine-cytokine receptor interaction, Jak-STAT signaling pathway
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Other "IL12RB1" proteins (6)
SKU Description Size Price
PH321974 IL12RB1 MS Standard C13 and N15-labeled recombinant protein (NP_005526) 10 ug
$3,360.00
LC403516 Lyophilized IL12RB1 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LC417240 Lyophilized IL12RB1 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LY403516 Transient overexpression lysate of interleukin 12 receptor, beta 1 (IL12RB1), transcript variant 2 (as WB positive control) 100 ug
$436.00
TP321974 Recombinant protein of human interleukin 12 receptor, beta 1 (IL12RB1), transcript variant 1, 20 µg 20 ug
$867.00
TP761820 Purified recombinant protein of Human interleukin 12 receptor, beta 1 (IL12RB1), transcript variant 2, full length, with N-terminal GST and C-terminal His tag, expressed in E. coli, 50ug 50 ug
$270.00

file-alt Citations

Probing the binding of interleukin-23 to individual receptor components and the IL-23 heteromeric receptor complex in living cells using NanoBRET
Lay, Bridges, Goulding et al
Cell Chem Biol (2021) 29 (1), 19-29.e6 DOI: 10.1016/j.chembiol.2021.05.002
Cited by 14
AI Summary: OriGene contributed IL23R-MycDDK expression plasmid (Cat# RC211477) and IL12Rβ1 expression plasmid (Cat# SC303661), which were used to express the IL-23 receptor components for binding studies in this paper.
Snippet: ynthesis IL6SS-NL-IL23R Genscript Custom synthesis IL6SS-HT-TEV-IL12Rβ1 Genscript Custom synthesis IL6SS-HT-TEV-IL23R Genscript Custom synthesis IL23R-MycDDK expression plasmid Origene Cat# RC211477 IL12Rβ1 expression plasmid Origene Cat# SC303661 pc 3.1 zeo SigSNAP-tag (Gherbi et al., 2015) Custom synthesis pc3.1 zeo Invitrogen Cat# V86020 ------------;IL23R-MycDDK expression plasmid Origene Cat# RC211477 IL12Rβ1 expression plasmid Origene Cat# SC303661
Showing 1-1 of 1 papers.
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