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NGAL (LCN2) (NM_005564) Human Over-expression Lysate

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SKU
LY417226
Transient overexpression lysate of lipocalin 2 (LCN2) (as WB positive control)
  $436.00
5 Days*
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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC207685
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_005564, NP_005555
Synonyms 24p3; MSFI; NGAL; p25
Predicted MW 22.6 kDa
Components 1 vial of 100 µg total protein derived from gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
Storage The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 1x SDS-PAGE running buffer (with reducing agent). Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C.
Shipping Dry Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.).
Reference Data
RefSeq NP_005555
Locus ID 3934
Cytogenetics 9q34.11
Protein Families WB
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Other "NGAL" proteins (7)
SKU Description Size Price
PH307685 LCN2 MS Standard C13 and N15-labeled recombinant protein (NP_005555) 10 ug
$3,360.00
LC417226 Lyophilized LCN2 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
TP307685 Recombinant protein of human lipocalin 2 (LCN2), 20 µg 20 ug
$737.00
TP701117 Purified recombinant protein of Human lipocalin 2 (LCN2), full length, with C-terminal Myc-DDK tag, secretory expressed in HEK293 cells, 50ug 50 ug
$900.00
TP721163 Purified recombinant protein of Human lipocalin 2 (LCN2) 10 ug
$260.00
TP723847 Purified recombinant protein of Human lipocalin 2 (LCN2 / NGAL) 10 ug
$345.00
TP762367 Purified recombinant protein of Human lipocalin 2 (LCN2), Gln21-End, with N-terminal His tag, expressed in E.coli, 50ug 50 ug
$260.00

file-alt Citations

Mutant p53 Together with TGFβ Signaling Influence Organ-Specific Hematogenous Colonization Patterns of Pancreatic Cancer
Zhong, Macgregor-Das, Saunders et al
Clin. Cancer Res. (2016)
AI Summary: OriGene products, including mouse COL6A1 (TF500404, MG223027), mouse Lcn2 (TR511895, MR226233), and a scrambled shRNA control vector (TR30015), were used in this study to manipulate gene expression and investigate the roles of these genes in pancreatic cancer metastasis.
Snippet: mouse COL6A1 or mouse Lcn2 (OriGene, TF500404 and TR511895) as well as a Non- effective 29-mer scrambled shRNA cassette in pRFP-C-RS Vector (OriGene, TR30015) ... expressing mouse COL6A1 or Lcn2 (OriGene, MG223027 and MR226233). The Trp53 ... Cite SaveSaving
Showing 1-1 of 1 papers.
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