OriGene Logo

Customer Login

Checkout as a new customer

Creating an account has many benefits:

  • See order and shipping status
  • Track order history
  • Check out faster
Create an Account

TRIP (TRAIP) (NM_005879) Human Over-expression Lysate

Be the first to review this product
SKU
LY416999
Transient overexpression lysate of TRAF interacting protein (TRAIP) (as WB positive control)
  $436.00
5 Days*
Bulk/Customize

Frequently Bought Together (3)

TA800086 TRAIP (TRIP) mouse monoclonal antibody, clone OTI2D4 (formerly 2D4) 100 ul
$447.00
TA811000S beta Actin Mouse Monoclonal Antibody, Clone OTI1, Loading Control 30 ul
$200.00
TA592569 DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
100 ul
$519.00

Other products for "TRIP or NM_005879"

Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC201252
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_005879, NP_005870
Synonyms RNF206; SCKL9; TRIP
Predicted MW 53.3 kDa
Components 1 vial of 100 µg total protein derived from gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
Storage The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 1x SDS-PAGE running buffer (with reducing agent). Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C.
Shipping Dry Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.).
Reference Data
RefSeq NP_005870
Locus ID 10293
Cytogenetics 3p21.31
Protein Families WB
Reviews
Write Review
 
 
 
 
 

Be the first to review this product

Please note:
Only reviews with images are eligible for a $20/20€ Amazon gift card.
Reviews without images are eligible for a $10/10€ Amazon gift card.
For more details about the terms and conditions, please visit the promotion page.

Review image
Documents
Resources
Other "TRIP" proteins (1)
SKU Description Size Price
LC416999 Lyophilized TRAIP HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00

file-alt Citations

The TRAF-interacting protein (TRAIP) is a regulator of the spindle assembly checkpoint
Chapard, Meraldi, Gleich et al
J. Cell. Sci. (2014)
Cited by 31
AI Summary: In this study, OriGene's Control siRNA was used as a negative control in RNA interference experiments to assess the specific effects of TRAIP depletion on mitotic progression and spindle assembly checkpoint regulation.
Snippet: 228 229 RNA interference 230 siRNA targeting human TRAIP mRNA (NM_005879.2) and Control siRNA (Origene), 231 hCYLD (Draviam et al., 2007;Sun et al., 2010), and hMAD2 (Uzunova et al., 2012) were 232 transfected ... Cite SaveSaving
Showing 1-1 of 1 papers.
Powered by BizGenius
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Related Products